干扰素α-2b作为青光眼滤过术后辅助用药的实验研究

目的 探讨干扰素α－２ｂ（ＩＦＮ α－２ｂ）对结膜下瘢痕形成的抑制作用机理和青光眼滤过术后的应用方式。方法 采用免疫组织化学ＰＡＰ法检测ＩＦＮα－２ｂ对体外培养的牛结膜下成纤维细胞表皮生长因子（ＥＧＦ）受体的影响,并用ＩＢＡＳ图像分析系统定量分析;观察ＩＦＮα－２ｂ对３６只兔眼结膜下置线引起的瘢痕中胶原含量的影响及１８只兔眼小梁切除术后房水蛋白的变化。结果 ＩＦＮα－２ｂ具有减少成纤维细胞表达ＥＧ

A study of interferon alpha-2b as an accessory drug after glaucoma filtration surgery

OBJECTIVE: To elucidate the inhibitory action of interferon alpha-2b (IFN alpha-2b) on the cicatrization under conjunctiva and the best way to use it after glaucoma filtration surgery. METHODS: Immunohistochemical PAP method and image biological system 2000 (IBAS 2000) were utilized to quantitatively analyze the inhibitory effect of IFN alpha-2b on the expression of epidermal growth factor receptor (EGFR) of cultured calf fibroblasts; the effects of IFN alpha-2b on collagen contents in suture-caused cicatrization under conjunctiva and changes of aqueous humor protein after trabeculectomy performed on rabbits have also been observed. RESULTS: IFN alpha-2b reduced the expression of EGFR of cultured bovine fibroblasts at a dose-dependent manner. The relationship between the EGFR and the dosage of IFN alpha-2b is Y = 2953.8 - 554.5 lgx (r = -0.98, P < 0.01). With 4.67 x 10(-5) micromol/L IFN alpha-2b not only reduced about 50% of the collagen in suture-caused cicatrization, but also significantly decreased the protein concentration of aqueous humour 3 and 72 hours after trabeculectomy. CONCLUSION: It is promising that IFN alpha-2b can be used after glaucoma filtration surgery to reduce excessive cicatrization with many advantages compared with antimetabolites.