细胞生长因子对人晶体上皮细胞转铁蛋白mRNA表达的影响

目的 分析转化生长因子－β２和碱性成纤维细胞生长因子对培养的人晶体上皮细胞转铁蛋白ｍＲＮＡ表达的影响,方法 人晶体前囊膜取和于３岁以下各种原因死亡２４ｈ内的２０例（４０只眼）婴和眼球。晶体上皮细胞原代培养和传代培养,选用传代汇合后２周的细胞,分别用ＴＧＦ－β１和ｂＦＧＦ作用天,用异硫氰酸胍、酚、氯仿、异戊醇等抽提细胞总ＲＮＡ,选择转铁蛋白的核酸探针进行Ｎｏｒｔｈｅｒｎ杂交,分析转铁蛋白ｍＲＮＡ的表

Influence of cell growth factors on transferrin gene expression of human lens epithelial cells in culture

Objective To assess the influence of transforming growth factor1 (TGF1) and basic fibroblast growth factor (bFGF) on the transferrin mRNA levels of cultured human lens epithelial cells. Methods Lens anterior capsules were collected from 40 eyeballs of 20 human donors under 3 years of age within 24 hours after death. Lens epithelial cells were primarily cultured and subcultured. TGF1 (1 g/L) and bFGF (250 g/L) were added to the 3rd passage cells when they became confluent after their growth for 2 weeks. The total cellular RNA was extracted from cultured cells by guanidine isothiocyanate, etc. after the growth factors acting for 3 days. Transferrin mRNA levels were determined by a Northern blot analysis with a probe. Results In comparison with the control group, the expressions of transferrin in the growth factor treatment groups were obviously increased. In comparison with bFGF group, transferrin mRNA level in TGF1 group was obviously increased. Conclusions TGF1 and bFGF upregulate transferrin gene expression of cultured lens epithelial cells remarkably, can reduce lenticular oxidative damaging process in which transferrin participates and play an important role in the pathological mechanism of cataract formation after extracapsular cataract extraction.