视网膜表面膜的超微结构特征

目的 探讨视网膜收缩膜细胞与胶原作用的超微结构特征及细胞外间质的作用。方法 对１６例孔源多膜脱离伴增殖性玻璃体视网膜病变（ｐｒｏｌｉｆｅｒａｔｉｖｅ ｖｉｔｒｅｏｒｅｔｉｎｏｐａｔｈｙ,ＰＶＲ）患者行玻璃体切除术,并应用免疫胶体金标记技术对术中获得的视网膜表面膜进行细胞外间质（ｅｘｔｒａｃｅｌｌｕｌａｒ ｍａｔｒｉｘ,ＥＣＭ）－－Ⅰ和Ⅲ型胶原及纤维粘连蛋白（ｆｉｂｒｏｎｅｃｔｉｏｎ,ＦＮ）的免疫电

Ultrastructural characteristics of epiretinal membranes

OBJECTIVE: To investigate ultrastructural features and the correlation between the cell activation and extracellular matrix (ECM) expression of epiretinal membranes. METHODS: Immuno-electron-microscopy was done on the epiretinal membranes removed by vitrectomy from 16 patients with rhegmatogenous retinal detachment associated with proliferative vitreoretinopathy (PVR). The specimens were stained with antibodies to ECM proteins, including type I collagen, type III collagen and fibronectin (FN). RESULTS: A type of cells with a number of lamellipodia was found in membrane sections of 9 patients. The lamellipodia appeared to be either coiled or branched and were surrounded by abundant collagen fibers of type I and III. The cells with lamellipodia varied in their phenotypes and with or without pigment. There were dense gold particles of FN in contact regions of lamellipodia and collagen. CONCLUSIONS: Our observations support that the contraction of epiretinal membranes is the result of an interaction between cells and collagen, and the cells extending lamellipodia may initiate contractile events. FN is responsible for this contractile action.