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人骨髓间充质干细胞体外定向分化为心肌细胞的实验研究
引用本文:徐长宪,许运宾,郭兰敏,张永善,孟国伟. 人骨髓间充质干细胞体外定向分化为心肌细胞的实验研究[J]. 山东大学学报(医学版), 2005, 43(9): 863-864,867
作者姓名:徐长宪  许运宾  郭兰敏  张永善  孟国伟
作者单位:山东大学医学院,山东,济南,250012;山东大学第二医院心脏外科,山东,济南,250033
摘    要:目的:建立人骨髓间充质干细胞(MSCs)体外定向分化为心肌细胞的实验模型。方法:取6个月水囊引产胎儿股骨骨髓,Percoll分离液分离,取单个核细胞层在人MSCs专用MSCGM培养基中体外培养。将第3代生长状态良好的MSCs用10μmol/L 5-氮胞苷孵育诱导24h,继续向心肌细胞诱导分化。结果:用Percoll分离液(1.073g/ml)分离得到的单个核细胞48~72h呈纺锤形、梭形、多角形的多型性改变;经5-氮胞苷孵育24h,细胞逐渐向梭形心肌细胞样形态转变,胞浆中可见棕黄色颗粒。结论:MSCs在体外经5-氮胞苷孵育24h,可被定向诱导分化为心肌形态的细胞。

关 键 词:骨髓间充质干细胞  细胞分化  心肌细胞
文章编号:1671-7554(2005)09-0863-02
收稿时间:2005-05-10
修稿时间:2005-05-10

Experimental study on the induced differentiation of human bone marrow mesenchymal stem cells into cardiomyocytes in vitro
XU Chang-xian,XU Yun-bin,GUO Lan-min,ZHANG Yong-shan,MENG Guo-wei. Experimental study on the induced differentiation of human bone marrow mesenchymal stem cells into cardiomyocytes in vitro[J]. Journal of Shandong University:Health Sciences, 2005, 43(9): 863-864,867
Authors:XU Chang-xian  XU Yun-bin  GUO Lan-min  ZHANG Yong-shan  MENG Guo-wei
Affiliation:1.School of Medicine, Shandong University, Jinan 250012, Shandong, China; 2.Department of Cardiovascular Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China
Abstract:Objective: To establish a model of human mesenchymal stem cells (MSCs) differentiated into cardiomyocytes in vitro. Methods: Bone marrow samples were derived from bone marrow of a 6-month fetus aborted with water bag. Mononuclear cells were separated and isolated by gradient centrifugation in 1.073 g/ml Percoll solution. Then they were seeded and expanded in specific human MSCs medium. MSCs in third passage were co-cultured and treated with 10 mmol/L 5-aza for 24 hours. Results: Mononuclear cells adhered to the flasks and grew typically in fibroblastic or spindle shape. Multifoot cells changed into orange-like ones with a spindle shape as that of cardiomyocytes. By immmunocytochemistry, they were found to be positively stained with actin. Conclusion: Human MSCs can be induced to differentiate into cardiomyocytes by 5-azacytidine in vitro .
Keywords:Bone marrow mesenchymal stem cells   Cell differentiation   Cardiomyocytes
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