| 高效液相色谱测定尿中香草扁桃酸含量 |
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| 摘 要： |
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| 关 键 词： | HPLC 香草扁桃酸 尿检测 |
Determination of vanillymandelic acid in urine by high performance liquid chromatography] |
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| L M Zheng,Y Tang,T Shen. Determination of vanillymandelic acid in urine by high performance liquid chromatography][J]. Bulletin of Hunan Medical University, 2000, 25(5): 492-494 |
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| Authors: | L M Zheng Y Tang T Shen |
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| Affiliation: | Department of Medical Laboratory, Xiangya Hospital, Hunan Medical University, Changsha 410008. |
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| Abstract: | OBJECTIVE: To develop a high performance liquid chromatography(HPLC) for determining vanillylmandelic acid(VMA) in urine. METHODS: The analytical column was packed with Nova-Pak C18. Flow rate was 0.9 ml.min-1. The mobile phase was 40 mmol.L-1 NaH2PO4 buffer. The VMA of 30 healthy people in 24 hour urine were tested by the internal standard method and with electrochemical detector. RESULT: The linear range was from 5 mumol.L-1 to 200 mumol.L-1. The mean analytical recovery rate was 99.1%. The mean within-day coefficient of variation(CV) was 5.8%, and the mean day-to-day CV was 7.7%. The detection limit was 0.28 mumol.L-1. The VMA of 24 hour urine was 18.13 +/- 6.17 mumol in healthy people. CONCLUSIONS: The method is suitable for routine determination of VMA in urine. |
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