TaqMan聚合酶链反应技术检测结核分支杆菌DNA及其临？…

目的 探讨ＴａｑＭａｎ聚合酶链反应（ＴａｑＭａｎ－ＰＣＲ）技术在肺结构诊断中的价值。方法 对１６８例活动性肺结核、５７例肺癌患者的痰和外周血及３４－例健康对照外周血，同时应用ＴａｑＭａｎ－ＰＣＲ、ＰＣＲ检测，并与痰涂片法、ＢＡＣＴＥＣ法及改良罗氏培养法结果进行比较。结果 ＴａｑＭａｎ－ＰＣＲ检测痰和外周血总的阳性率分别为５３．０％和６１．３％，显著高于ＰＣＲ、痰涂片法、ＢＡＴＣＴＥＣ法及改良罗氏培

Detcetion of DNA extracted from Mycobacterium tuberculosis by TaqMan-PCR technique and its clinical application

Objective To evaluate the clinical diagnostic value of TaqMan PCR technique in the diagnosis of active pulmonary tuberculosis Methods The specimens of sputum and peripheral blood from 168 patients with active pulmonary tuberculosis, 57 patients with lung cancer and the specimens of peripheral blood from 34 healthy individuals were detected by TaqMan PCR, PCR The specimens of sputum were also detected by smear, culture with BACTEC and Lowenstein Jensen for comparison Results The positive rates of sputum and peripheral blood detected by TaqMan PCR were 53 0% and 61 3%, respectively, significantly higher than those of PCR, smear, culture with BACTEC and Lowenstein Jensen ( P <0 05) There was no significant difference between TaqMan PCR and PCR for specificity ( P >0 05) Of 105 specimens of peripheral blood from patients with smear negative and culture negative pulmonary tuberculosis, 48 specimens of peripheral blood detected by TaqMan PCR were positive The positive rate was 45 7% Conclusions TaqMan PCR method showed higher sensitivity and specificity It is an useful tool for diagnosis of pulmonary tuberculosis, particularly smear negative and culture negative pulmonary tuberculosis