Heterodimeric complexes of Hop2 and Mnd1 function with Dmc1 to promote meiotic homolog juxtaposition and strand assimilation |
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Authors: | Chen Yi-Kai Leng Chih-Hsiang Olivares Heidi Lee Ming-Hui Chang Yuan-Chih Kung Wen-Mei Ti Shih-Chieh Lo Yu-Hui Wang Andrew H-J Chang Chia-Seng Bishop Douglas K Hsueh Yi-Ping Wang Ting-Fang |
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Affiliation: | Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan. |
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Abstract: | Saccharomyces cerevisiae Hop2 and Mnd1 are abundant meiosisspecific chromosomal proteins, and mutations in the corresponding genes lead to defects in meiotic recombination and in homologous chromosome interactions during mid-prophase. Analysis of various double mutants suggests that HOP2, MND1, and DMC1 act in the same genetic pathway for the establishment of close juxtaposition between homologous meiotic chromosomes. Biochemical studies indicate that Hop2 and Mnd1 proteins form a stable heterodimer with a higher affinity for double-stranded than single-stranded DNA, and that this heterodimer stimulates the strand assimilation activity of Dmc1 in vitro. Together, the genetic and biochemical results suggest that Hop2, Mnd1, and Dmc1 are functionally interdependent during meiotic DNA recombination. |
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