PAI-1基因多态性与包头地区汉族2型糖尿病并发肾病的相关性研究

目的：探讨血浆纤溶酶原激活物抑制剂-1（plasminogen activator inhibitor-1,PAI-1）基因多态性与2型糖尿病肾病（diabetic nephropathy,DN）的相关性,并初步研究其可能机制。方法：50例正常对照组,120例2型糖尿病,测定任意尿白蛋白与肌酐比值（Ua/c）,根据比值将糖尿病组分为50例糖尿病非肾病组、70例糖尿病肾病组。提取DNA,聚合酶链反应扩增,产物以琼脂糖凝胶电泳分离确定PAI-1基因型。并收集体重、身高、血压、血糖、糖化血红蛋白、甘油三酯、胆固醇等资料。结果：（1）正常人、糖尿病非肾病与糖尿病肾病组相比,PAI-14G/4G基因型的分布有明显的差别,糖尿病肾病组PAI-1基因中4G等位基因频率明显高于糖尿病非肾病组（0.550∶0.149,χ^2=35.685,P〈0.05）。（2）Logistic回归分析表明PAI-1基因是2型糖尿病肾病的独立患病危险因素。PAI-14G/4G基因型比其他基因型具有更高的糖尿病肾病发病危险。结论：2型糖尿病肾病组PAI-1基因4G/4G型和4G等位基因频率明显高于正常人、2型糖尿病非肾病组。PAI-1基因4G/4G型是2型糖尿病肾病发病的独立患病危险因素。PAI-14G/4G型为糖尿病肾病发病的易感性基因。

Polymorphisms of plasminogen activator inhibitor-1 genes in type 2 diabetes with nephropathy in han in baotou

Objective:To investigate the frequency and functional significance of plasminogen activator inhibitor-1(PAI-1)gene.Methods:PAI-1 gene in the group of 50 normal subjects and 120 type 2 diabetes were detected by means of polymerase chain reaction(PCR).According to measurement of the albumin-to-creatinine ratio(ACR)of free urine,the group of type 2 diabetes were devided to diabetic nephropathy positive(DN+)group and diabetic nephropathy negative(DN-)group.Weight,height,arterial blood pressure,total cholesterol...