| 涎腺干/祖细胞长期传代后染色体核型分析 |
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| 引用本文： | 胡小华,黄桂林,姜群,张霓霓.涎腺干/祖细胞长期传代后染色体核型分析[J].口腔医学研究,2010,26(4):512-514. |
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| 作者姓名： | 胡小华 黄桂林 姜群 张霓霓 |
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| 作者单位： | 1. 遵义医学院附属口腔医院口腔颌面外科,贵州遵义,560003
2. 威海市口腔医院口腔外科 |
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| 基金项目： | 贵州省科学技术厅科技攻关项目(2003)53号贵州省优秀科技教育人才省长基金(黔省专合字(2008)113号贵州省教育厅基金项目(2006)354号 |
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| 摘 要： | 目的：分离主导管结扎后损伤下颌下腺组织中的干/祖细胞（Salivary Gland Progenitor,SGP）,进行长期培养,对10～25代的染色体进行分析。方法：结扎SD大鼠下颌下腺主导管,获得损伤模型,机械及酶消化法体外分离培养腺体细胞,获得类上皮细胞集落,挑取集落后梯度稀释法纯化获得类上皮单克隆细胞-涎腺干/祖细胞,对长期培养的SGP行染色体常规及G-带核型分析。结果：SGP染色体数2n=42,其中常染色体20对,性染色体1对;SGP染色体数目、形态及结构未发现特异性异常改变。结论：长期传代培养的SGP二倍体性状不改变,遗传性状相对稳定。
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| 关 键 词： | 涎腺干/祖细胞 染色体 核型分析 |
Analysis of Chromosome Karyotype of Salivary Glande Progenitor Cells Induced by Tissue Trauma |
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| Institution: | HU Xiao-hua,HUANG Gui-lin,JIANG Qun,et al.Affiliated Stomatological Hospital,Zunyi Medical College,Zunyi 560003 |
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| Abstract: | Objective：To isolate salivary gland progenitor cells from damaged submandibular gland and analyze chromosome G-banding karyotype by long-term culture.Methods：Tissue trauma was performed by ligation of main duct of submandibular gland in Sprague-Dawlye（SD）rats.Cells were isolated by mechanical method and enzyme digestion.With limited dilution,cell line purified from epithelium-like colony was designated as salivary gland progenitor cells and G-banding karyotype was analyzed by long-term culture.Results：The chromosome number of SGP cell line was 2n=42with no abnormality in number,morphology and structure.Conclusion：After long time culture,the SGP cell line maintained its digenomous diploid without abnormality so as to prove their inheritancestability. |
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| Keywords: | Salivary gland progenitor cells（SGP） Chromosome Karyotype |
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