| HPLC—MS法和mFPIA法测定全血环孢霉素A及其代谢物的比较 |
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| 引用本文： | 王彬 娄子洋 柴逸峰 张国庆. HPLC—MS法和mFPIA法测定全血环孢霉素A及其代谢物的比较[J]. 药物分析杂志, 2007, 27(9): 1378-1381 |
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| 作者姓名： | 王彬 娄子洋 柴逸峰 张国庆 |
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| 作者单位： | 第二军医大学东方肝胆外科医院药材科，第二军医大学药学院 |
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| 基金项目： | 第二军医大学青年启动基金资助（No.05QN42） |
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| 摘 要： | 目的:对测定全血环孢霉素 A 的 HPLC-MS 法和单克隆荧光偏振免疫分析法(mFPIA 法)进行评价。方法:建立HPLC-MS 测定全血环孢霉素 A 及其代谢物的方法,采用多元线性回归对 HPLC-MS 法和 mFPIA 法测定的结果进行比较。结果:当患者肝、肾功能异常时,mFPIA 法测定结果同环孢霉素 A 及主要代谢物浓度呈线性相关,易导致检测结果偏高。结论:当患者肝、肾功能异常时,mFPIA 法不适宜作为环孢霉素 A 的临床监测方法。
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| 关 键 词： | 环孢霉素 A HPLC-MS 单克隆荧光偏振免疫分析法(mFPIA 法) 多元线性回归 |
| 文章编号： | 0254-1793（2007）09-1378-04 |
| 修稿时间： | 2007-07-20 |
Comparison of monitoring of human whole -blood levels of cyclosporin A and its metabolites by HPLC- MS and mFPIA methods |
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| WANG Bin, LOU Zi - yang, CHAI Yi - feng, ZHANG Guo - qing. Comparison of monitoring of human whole -blood levels of cyclosporin A and its metabolites by HPLC- MS and mFPIA methods[J]. Chinese Journal of Pharmaceutical Analysis, 2007, 27(9): 1378-1381 |
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| Authors: | WANG Bin LOU Zi - yang CHAI Yi - feng ZHANG Guo - qing |
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| Affiliation: | 1. Department of Pharmacy, Eastern Hepatobilliary Hospital, The Second Military Medical University, Shanghai 200438, China; 2. College of Pharmacy,The Second Military Medical University,Shanghai 200433 ,China |
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| Abstract: | Objective:To compare a determination for cyclosporin A by HPLC-MS and monoclone fluorescenc polarization immunoassay(mFPIA).Methods:To establish a determination for cyclosporin A and its metabolites i human whole-blood by HPLC-MS and to compare results determined by both HPLC-MS and mFPIA with multi ple linear regression.Results:When liver and kidney dysfunctions of patients were abnormal,concentration of cy closporin A determined by mFPIA had a linear relation with its main metabolites.Conclusion:mFPIA is not suitabl for patients with liver and kidney dysfunctions. |
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| Keywords: | cyclosporin A HPLC-MS mFPIA multiple linear regression |
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