| 高效液相色谱法测定癌痛宁巴布剂中乌头碱的含量 |
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| 引用本文： | 夏崇才,高运军,方明治,周芙琼. 高效液相色谱法测定癌痛宁巴布剂中乌头碱的含量[J]. 中国医药导报, 2013, 0(31): 112-114 |
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| 作者姓名： | 夏崇才 高运军 方明治 周芙琼 |
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| 作者单位： | [1]江苏省南京市中医院中医药研究所,江苏南京210001 [2]江苏省南京市中医院肿瘤科,江苏南京210001 |
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| 基金项目： | 江苏省中医药局中医药科技课题（编号LZ11101）. |
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| 摘 要： | 目的建立癌痛宁巴布剂中乌头碱的含量测定方法。方法高效液相色谱（HPLC）法，采用Kromasil C18柱（4．6mm×250mm），流动相为甲醇-0．2％三乙胺（66：34），测定波长为230nm，流速为1．0mL／min，柱温为30℃。结果乌头碱在0．126-0．630μg的范围内的范围内线性关系良好，r=0．9999，平均回收率为97．47％，RSD为1．2％。结论方法简便可行，重现性好．可用于癌痛宁巴布剂的质量控制。
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| 关 键 词： | 乌头碱 癌痛宁巴布剂 高效液相色谱法 含量测定 |
Determination of Aconitum in Aitongningbabu Plaster by HPLC |
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| XIA Chongcai,GAO Yunjun,FANG Mingzhi,ZHOU Fuqiong. Determination of Aconitum in Aitongningbabu Plaster by HPLC[J]. China Medical Herald, 2013, 0(31): 112-114 |
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| Authors: | XIA Chongcai GAO Yunjun FANG Mingzhi ZHOU Fuqiong |
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| Affiliation: | 1.TCM Research Institution, Nanjing Hospital of TCM, Jiangsu Province, Nanjing 210001, China; 2.Department of Oncology, Nanjing Hospital of TCM, Jiangsu Province, Nanjing 210001, China) |
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| Abstract: | Objective To establish a method for the determination of Aconitum in Aitongningbabu Plaster. Methods HPLC analytical method was established. The separation was performed on Kromasil C18 column （4.6 mm×250 mm）, the column temperature was 30℃ using methanol -0.2% triethylamine （66：34） as mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was at 230 nm. Results The linear range of Aconitum was in the range of 0.126-0.630μg, r = 0.9999, the average recovery rate was 97.47% and RSD was 1.2%, respectively. Conclusion This method is simple, rapid and accurate and can be used as a quantitative analysis for this preparation. |
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| Keywords: | Aconitum Aitongningbabu Plaster HPLC Content determination |
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