常见恶性肿瘤新鲜组织中原发性多药耐药基因表达的初步研究

目的从基因水平探讨临床常见恶性肿瘤细胞中原发性多药耐药基因（ｍｄｒ－１）的表达规律,指导临床实践。方法取安阳市肿瘤医院１９９４年１１月～１９９５年９月术前未做治疗的恶性肿瘤切除标本１５１份,以反转录多聚酶链反应法（ＲＴ－ＰＣＲ）检测其ｍｄｒ－１基因的表达情况,并做比较研究。结果１５１例均经病理检查证实为恶性肿瘤,包括胃及贲门癌５１例,食管癌４６例,大肠癌１６例,乳腺癌１５例,甲状腺癌１０例,肺癌９例,宫颈癌４例。ｍｄｒ－１基因在上述肿瘤中的阳性表达率依次分别为３３．３％、３７．０％、３１．３％、１３．２％、４０．０％、５５．５％、０。结论ＲＴ－ＰＣＲ检测肿瘤组织中ｍｄｒ－１基因的表达简单方便、可靠准确。ｍｄｒ－１基因在临床常见恶性实体肿瘤组织中有较高的原发性表达,提示化疗时要区别对待,合理选药。

Inherent mdr-1 gene expression in fresh tumor tissue specimens from several high-incidence malignancies

Objective To ascertain frequency of inherent mdr 1 gene expression in several common neoplasms. Methods One hundred fifty one surgically resected tumor specimens confirmed pathologically to be malignant from preoperatively untreated cancer patients were studied. Expression of mdr 1 gene was examined by RT PCR. Results The frequency of primary mdr 1 gene expression was 33.3% for cancer of the stomach and gastric cardia (17/51), 37% for cancer of the esophagus (17/46), 31.3% for cancer of the colon and rectum (5/16), 13.2% for cancer of the breast (2/15), 40.0% for cancer of the thyroid (4/10), 55.0% for cancer of the lung (5/9) and 0% for cancer of the uterine cervix (0/4). Conclusion Primary mdr 1 gene expression is relatively of frequent occurrence as detected by RT PCR technique.