应用末端氧核苷酰转移酶法研究化疗药诱导人肿瘤细胞的凋亡

目的 应用末端脱氧核苷酰转移酶尘（ＴｄＴ法）研究抗药物诱导人肿瘤细胞的凋亡,方法 应用ＴｄＴ法、ＤＮＡ凝胶电泳以及电镜、光镜等技术研究有关细胞的凋亡。结果 本研究表明,顺铂可以诱地人白血病的ＨＬ－６０细胞和经ＳＶ４０－Ｔ转染的人支气管上皮细胞的雕恨,并有很好的剂量和时间反应,同时还观察到,这些细胞的ｂｃｌ－２ｍＲＮＡ表达降低先于细胞凋亡的发生。应用ＴｄＴ法检测,在卡铂治疗的卵巢癌患者的肿瘤细胞中到

Study on antitumor drug-induced apoptosis in human cancer cells by terminal deoxynucleotidyl transferase assay

OBJECTIVE: Considerable evidence has showed that apoptosis is involved in both cancer development and inhibition. A new assay (terminal deoxynucleotidyl transferase assay, TdT assay) was recently reported to have advantages in the detection of apoptosis. In this study, this assay was used to investigate antitumor drug-induced apoptosis in human cancer cells. METHODS: TdT assay, DNA gel electrophoresis, electron and light microscopy were used to observe apoptosis. RESULTS: Our results showed that cisplatin-induced apoptosis in both HL-60 and SV40T-transformed human bronchial epithelial cells was detected with a good dose and time response. The occurrence of the apoptosis was preceded by the decrease of bcl-2 mRNA expression. With TdT assay, apoptotic cells were observed in the ovarian tumor of patients treated with carboplatin. CONCLUSION: TdT assay may be applicable to moniter apoptosis in human cancers induced by chemotherapy, and to evaluate tumor cell response during treatment.