导入H—ras癌基因后肝癌细胞转移特性的变化

目的探讨Ｈ－ｒａｓ癌基因与肝癌细胞转移行为的关系。方法应用磷酸钙转染法将Ｈ－ｒａｓ癌基因导入人肝癌细胞株ＳＭＭＣ７７２１,通过进行粘附试验、运动试验、Ⅳ型胶原酶（ｃⅣａｓｅ）的分泌、表皮生长因子受体（ＥＧＦＲ）的表达等转移相关指标的测定,观察转染前后细胞转移特性的变化。结果转染Ｈ－ｒａｓ的ＳＭＭＣ７７２１细胞对胞外基质成分层粘连蛋白（ＬＮ）、纤维粘连蛋白（ＦＮ）的粘附能力提高,细胞的运动能力增强,ｃⅣａｓｅ的分泌明显增加,ＥＧＦＲ表达有中等程度的增加,且上述变化与Ｈ－ｒａｓ癌基因的蛋白产物ｐ２１的表达有明确的相关关系。结论Ｈ－ｒａｓ癌基因能在体外诱导人肝癌细胞的转移表型的产生,提高肝癌细胞的转移潜能。

Alterations in metastatic properties of hepatocellular carcinoma cell following H-ras oncogene transfection

OBJECTIVE: To demonstrate the relationship between H-ras oncogene and HCC metastasis. METHODS: Activated H-ras oncogenes were transfected into SMMC7721, a cell line derived from human hepatocellular carcinoma (HCC), by calcium phosphate transfection method. Some metastasis-related parameters were detected in vitro, including adhesion assay, migration assay, expresion of collagenase IV (cIVase) and EGFR. RESULTS: The abilities of H-ras-transfected cell clones in adhesion to laminin or fibronectin, migration, cIVase secretion increased markedly, and the expression of EGFR elerated moderately. More importantly, these alterations were consistently positive with the expression of p21, the protein product of H-ras oncogene. CONCLUSION: H-ras oncogene could induce the metastatic phenotype of HCC cell in vitro, therefore enhancing its metastatic potential.