肺癌患者FLK-1和LRP的实验与临床研究

目的：探讨血管内皮牛长凶子受体FLK-1和肺耐药蛋白LRP基因在肺癌发生发展过程中的作用。方法：用免疫组化技术（ABC法）对原发性肺癌组织中二种基因的表达进行检测。结果：70例肺癌中，非小细胞肺癌LRP过度表达率为69．5％，明显高于小细胞肺癌LRP过度表达率27．3％（P〈0．05）；腺癌中LRP的表达明显高于鳞癌（P〈0．05）。LRP与FLK-1在59例NSCLC中共同表达29例（49．2％），LRP的表达与肺癌的组织学分级、吸烟以及组织学类型有关；FLK-1与TNM分期相关，均有统计学意义（P〈0．05）：FLK-1＋LRP、中药治疗、复发与患者的预后有关（P〈0．05）。结论：FLK-1和LRP基因蛋白产物的检测对肺癌患者的诊治和预后评估有积极意义。

Experimental and clinical investigation of FLK-1 and LRP in human primary lung cancer

Objective:To investigate the effect of FLK-1 and LRP genes in the carcinogenesis and development of lung cancer.Methods:The expression of FLK-1 and LRP gene proteins in primary lung tumors was studied immunohistochemically.Results:Of the 70 lung cancers,the overexpression of LRP in NSCLCs was 69.5% and 27.3% in SCLCs,There was statistically significant correlation (P<0.05).The squamouscarcinoma was significantly different (P<0.05) from adenocarcinoma in the positive rate of LRP.The co-expression rate of LRP with FLK-1 in 59 cases NSCLCs was 49.2%.There was significant correlation between the expression of LRP and histological grade,smoke and histological type,the expression of FLK-1 and TNM staging (P<0.05).FLK-1,and LRP genes showed co-expression in some of the lung tumors.In a multivariate analysis by Cox regression model,the overexpression of FLK-1+LRP,the traditional Chinese Medical therapy and recurrence were independently associated with patient prognosis (P<0.05).Conclusion:The detection of FLK-1 and LRP geneproteins has positive effects on diagnosis,prognosis and treatment of lung cancer.