紫外分光光度法测定龙眼核提取物中总甾醇的含量

目的 建立龙眼核中总甾醇的含量测定方法.方法 采用紫外分光光度法,以磺基醋酸汞试剂为显色剂,豆甾醇为对照品,测定波长为410 nm.结果 样品在2.5 h内稳定,甾醇浓度在0.044～0.440 mg/mL具有良好的线性关系[相关系数（r）=0.999 9,n=5],平均回收率为99.5%,相对标准偏差（RSD）为2.52%（n=9）.结论 紫外分光光度法简便、快速、准确,可作为龙眼核中总甾醇的含量测定方法.

Determination of total sterol in longan seeds by UV spectrophotometry

Objective To establish a method for the determination of total sterol in longan seeds. Methods Uhraviolet（UV） spectrophotometry was used. The sulfonic mercuric acetate reagent was used as the chromogenie agent. The stigmasterol was taken as the reference substance. The detection wavelength was 410 nm. Results The absorption value was stable within 2.5 hours. The good linearity could be found when the concentration of sterol was in the range of 0.044-0.440 mg/mL [correlation coefficient （r）--0.999 9, n=5] with the average recovery rate of 99.5% and relative standard deviation （RSD） of 2.52% （n=9）. Conclusion UV spectrophotometry is accurate, simple and rapid for the determination of total sterol in longan seeds.