远志YZ-50对慢性应激抑郁模型大鼠海马Bax、Bcl-2表达的影响

目的：探讨远志YZ-50对慢性应激抑郁模型大鼠海马神经元凋亡因子Bax、Bcl-2表达的影响。方法：采用健康雄性Ⅱ级Wistar大鼠慢性应激抑郁模型，通过免疫组化染色法检测模型大鼠海马区凋亡调控因子Bax、Bcl-2蛋白的表达，从分子水平探讨在给予远志YZ．50后凋亡调控因子Bax、Bcl-2在慢性应激抑郁大鼠海马组织的变化规律。本实验共设五组，分别是空白对照组、慢性应激模型组、地昔帕明组（20mg·kg^-1）、YZ-50低剂量组（2．8g生药·kg^-1）、YZ-50高剂量（5．6g生药·kg^-1）组。结果：在给予大鼠慢性应激刺激21d，造成应激抑郁模型后，通过检测表明模型组动物Bax蛋白的表达明显增加，Bcl-2蛋白表达很弱；YZ-50低、高剂量组动物Bcl-2蛋白的表达与模型组动物相比明显增加；Bax蛋白的表达与模型组动物相比均显著降低（P〈0．05）。结论：YZ-50能够提高慢性应激抑郁模型大鼠海马区CA3区Bcl-2蛋白的表达、抑制Bax蛋白的表达，调控Bcl-2／Bax比例而抑制神经细胞的凋亡，减少外界刺激对脑部神经元的损害，从而改善其抑郁状态。

Effect of polygala tenuifolia willd YZ-50 on expression of the Bax,Bcl-2 of chronic stress depression rats

Objective： To investigate the effect of polygala tenuifolia willd YZ-50 on expression of the Bax, Bcl-2 of chronic stress depression rats. Methods： Expression of the Bax, Bcl-2 in the chronic stress depression rats＇ hippoeampus was detected by immunohistoehemistry methods with the healthy male Ⅱ grade wistar rats of chronic stress depression rats and the change rule of the Bax, Bcl-2 was discussed from molecular level. Experiment was allocated randomly to five groups： normal control group, model group, desipramine group （20 mg · kg^-1） and polygala tenuifolia willd YZ-50 low （2.8 g · kg^-1 dried medicinal herb） and high （5.6 g · kg^-1 dried medicinal herb） dosage groups. Results： When the chronic stress depression rats model was built successfully, results demonstrated that the expression of the Bax was increased obviously and the Bcl-2 expressed lowly in the model group; Compared with model group, the Bcl-2 showed up-regulation in each dosage of YZ-50, while the Bax decreased significantly （P 〈 0.05）. Conclusion： YZ- 50 increased the expression of Bcl-2, inhibited the expression of Bax, controlled the proportion of Bcl-2/Bax to reduce the apoptosis of the neurons and decreased the outside impaired effect towards neurons which improved the depressive state.