硒蛋氨酸诱导食管癌细胞株凋亡的实验研究

目的：探讨硒蛋氨酸对食管癌细胞系EC 9706凋亡的影响。方法：采用MTT比色法，细胞生长曲线描绘观察硒蛋氨酸对食管癌细胞系EC 9706增殖的影响。采用流式细胞仪观察硒蛋氨酸对EC 9706细胞诱导其凋亡的作用及对细胞周期的影响。琼脂糖凝胶电泳法检测DNA ladder。结果：硒蛋氨酸呈时间、剂量依赖性方式抑制EC 9706细胞增殖，改变细胞周期分布，增加G0／G1期细胞比例，诱导细胞凋亡。结论：硒蛋氨酸可能通过影响细胞周期分布和诱导细胞凋亡，从而抑制EC 9706细胞增殖。硒蛋氨酸可能是预防和治疗食管癌的一种新制剂。

A Study Apoptosis of Esophageal Cancer Cells Induced by Selenomethionine

Objective: The current study was designed to evaluate the potential role of selenomethionine for proliferation and apoptosis of esophageal cancer cell line EC 9706. Methods: MTT assay and growth curve of cells were used to determine the influence of selenomethionine on the proliferation of EC 9706 cells. Flow cytometry was used to observe the induction of selenomethionine on the apoptosis of EC 9706 cells and influence on the distribution cell cycle. DNA ladder were examined by DNA agarose gel electrophoresis. Results: Selenomethionine inhibited the cells proliferation in a time-and dose-dependent fashion, induced apoptosis of the cells in a does-dependent and increased the proportion of cell in the G0/G1 phase. Conclusion: Selenomethionine may inhibit the of esophageal cancer cells EC 9706 through effecting the distribution of cell cycle and inducing apoptosis. Selenomethionine may be a new way of chemoprevention and chemotherapy for esophageal cancer