生物材料补片修补大鼠左心室室壁瘤的实验研究

目的建立大鼠左心室室壁瘤补片模型，比较生物可降解材料p（3HB-co-3HH）／PU（PHB）与脱细胞牛心包片在该模型上的表现。方法取SD大鼠，结扎左冠状动脉，制成心梗模型。心梗模型制作6周后，行超声心动图检查，筛选合格模型大鼠，随机分成3组，PHB组（9只），脱细胞牛心包片组（9只），对照组（6只），前两组分别行左心室室壁瘤补片术，对照组仅行开胸手术。术后8周再次行超声心动图检查，并取补片做病理学检查。结果PHB组与脱细胞牛心包片组较对照组心功能明显改善（P〈0．01），但两组之间差异无统计学意义。大体病理显示两种补片局部均无室壁瘤形成，补片组织相容性好，心室面为内皮细胞覆盖。结论大鼠左心室室壁瘤补片模型可以满足进一步组织工程学心肌补片研究的需要，PHB补片与脱细胞牛心包片经过改进后可作为组织工程学心肌的支架材料。

Repair of left ventricular aneurysm with biomaterial patches in rats

Objective To establish an animal model and repair the left ventricular aneurysm in rats; compare biodegradable p(3HB- co-3HH)/PU patch with acellular pericardium in the model. Methods Six weeks after coronary ligation, Sprague-Dawley rats were assessed by echocardiography. Eligible animals were randomized into three groups, including the p(3HB-co-3HH)/PU patch group (n=9), the acellular pericardium patch group (n=9), and the control group (n=6). Both two patch groups were used for left ventricular aneurysm resection and repair, and the control group had only sham surgery. Eight weeks after the second surgical procedures, the survival animals were assessed by echocardiography again. Grafts histology and morphology were also studied.Results Heart function in both patch groups were significantly improved comparing with the control group (P<0.01), but there was no significant difference between the two patch groups. Morphology studies indicated that no ventricular aneurysm formed in the location of the patches, and histology studies showed that endothelial cells covered the ventricular surfaces of the patch.Conclusion The left ventricular aneurysm repair model in rats can satisfy the further studies of tissue engineering myocardium, and both p(3HB-co-3HH)/PU and acellular pericardium patches may be used as scaffold materials for tissue engineering myocardium studies after further improving.