幽门螺杆菌cagG基因在胃黏膜中的表达及其意义

目的 研究cagG基因在不同上消化道疾病患者中的分布及其与幽门螺杆菌(Helico-bacter pylori,Hp)感染相关疾病的关系。方法 合成特异性引物,应用聚合酶链式反应(PCR)法扩增145株分离培养自上消化道疾病患者的Hp菌株cagG片段,其中慢性胃炎72例,胃溃疡17例,十二指肠球部溃疡48例,复合溃疡8例。比较cagG阳性、阴性的胃黏膜炎症程度。结果 Hp菌株cagG片段总检出率为91.7％(133/145),在慢性胃炎、胃溃疡、十二指肠溃疡、复合性溃疡中cagG阳性率分别为90.3％。88.2％、93.8％和 100.0％,各组间差异无显著性意义:(P>0.05)。结论 cagG有较高的保守性,在不同的消化道疾病患者的Hp中均有较高的检出率,在不同疾病中的分布无特异性,与胃黏膜炎症程度无明显关系,cagG尚不能单独作为某种疾病致病的相关基因。

The cagG gene expression of Helicobacter pylori in various digestive diseases and its clinical significance

Objective To investigate the cagG gene expression of Helicobacter pylori isolated from patients with various digestive diseases and its relationship with gastroduodenal diseases. Methods The cagG fragments were amplified by polymerase chain reaction (PCR) in 145 Hp strains isolated from patients with chronic gastritis 72, gastric ulcer 17, duodenal ulcer 48, gastric and duodenal ulcer 8, and compared the differences of inflammation in gastric mucosa when cagG expression is positive or not. Results The cagG expression was positive in 91. 7% (133/145) Hp strains isolated from all the patients, and in patients with chronic gastritis, gastric ulcer, duodenal ulcer, both gastric and duodenal ulcer were 90.3% , 88.2% , 93. 8% , and 100. 0% respectively. There are statistically no significant differences among these four groups (P > 0. 05 ) . Conclusion The cagG gene of Hp was quite conservative, most of the Hp strains in Chinese patients were cagG positive, it has no specific predisposition to different disorders and shows no significant correlation to the extent of gastric mucosal inflammation. Therefore cagG can' t be served solely as a related pathogenic gene for certain diseases.