前S1蛋白和前S2蛋白及HBV-DNA平行检测对HBV感染诊断的临床价值

目的：探讨前S1蛋白和前S2蛋白联合诊断乙肝病毒感染复制的临床意义。方法：应用ELISA技术和PCR方法对216例HBsAg阳性标本进行前S1蛋白、前S2蛋白及HBV-DNA平行检测。结果：在“大三阳”组中，前S1蛋白、前S2蛋白及HBV-DNA检出阳性率分别为69．5％、42．9％和86．7％；在109例HBeAg阴性血清中，前S1蛋白39例（35．8％）、前S2蛋白5例（4．6％）及HBV-DNA14例（12．8％）检出阳性。前S1蛋白与HBV-DNA的阳性率比较差异无统计学意义（X^2=0.613 P〉0.250），与的阳性率比较差异无统计学意义（X^2=0.493，P〉0.250）。前S2蛋白与HBV-DNA阳性率比较差异有统计学意义（X^2=40．36，P〈0．005），与HBeAg阳性率比较差异有统计学意义（X^2=46．81，P〈0．005）。结论：前S1蛋白和前S2蛋白是诊断乙肝病毒感染复制十分有价值的血清学指标。

Clinical signification of detecting Pre-S1 protein and Pre-S2 protein in diagnosis of hepatitis B virus infection

Objective: To investigate the clinical significance of detection Pre-S1 protein and Pre-S2 protein in diagnosis of HBV infection. Methods:Using PCR to detect HBV-DNA and ELISA technique to detect Pre-S1 protein and Pre-S2 protein for 2i6 samples with HBsAg positive in the meantime. Results: Pre-S1 protein, Pre-S2 protein and HBV-DNA positive rates of sample with positive HBsAg, HBeAg, HBcAb markers were 69.5%, 42.9% and 86.7% respectively;Prs-S1 protein, Pre-S2 protein and HBV-DNA were positive in 39 (35.8%) cases, 5 (4.6%) cases and 14 (12.8%) cases in the 109 cases serum sample with negative HBeAg respectively. There was no significant difference between Pre-S1 protein and HBV-DNA (x2 = 0. 613,P>0.250). There was no significant difference between Pre-S1 protein and HBeAg by x2 test (x2=0.493, P>0.250). There was significant difference between Pre-S2 protein and HBV-DNA (x2 = 40. 36, P <0. 005),There was significant difference between Pre-S2 protein and HBeAg (x2=46. 81, P < 0.005). Conclusion: Pre-S1 protein and pre-S2 protein are both great applying valuably serological marker in clinical diagnosis of HBV infection and replication.