O139霍乱弧菌生物学特性及药物敏感性分析

为了有效、准确地检测霍乱弧菌，在某地水样泻患者的粪便中分离４株Ｏ１３９霍乱弧菌，经对分离株的生物学特性、血清学及药物敏感性进行研究，并与Ｏ１群霍乱弧菌进行比较。结果表明，Ｏ１３９霍乱弧菌具有Ｏ１群同样的生化特性，但不与Ｏ１群霍乱弧菌抗血清凝集，仅与Ｏ１３９霍乱弧菌抗血清在玻片上呈强凝集，试管凝集滴度为１：６４０～１：２５６０，所有菌株都使鸡红细胞凝集，对羊红细胞溶血的作用可变。所有菌株对霍乱Ｏ１群特异的ｍｕｋｈｅｒｊｅｅ＇ｓ噬菌体有抗性，对Ｏ／１２９多粘菌素Ｂ等药物耐药；而对红霉素、环丙氟哌酸等抗生素敏感。Ｏ１３９与Ｏ１群霍乱弧菌的不同特征，可作为鉴别的参考依据，但由于观察的菌株尚少，有待进一步研究。

Analysis on biological properties and drug sensitivity of V. cholerae Serogroup O139

strains of V.cholerae O1 3 9 isolated from waterystool of patients.Biologlcal properties and drug sensitivity. were studied and compared with those of V. cholerae O1.The results showed that V. cholerae O139 isoiates were charac terized by the same biochemical properties as that of V. cholerae O1. Serological characterization of those strains revealed that they only agglutinate with V,cholerae O1 39antiserum,but not to V. cholereae O1 antiserum. They agglutinated chiken erythrocytes on slides,but the result obtained by hemoly zation of sheep's erythrocytes were variable.All strains were resistant to Mukherjee's bacteriophage for both biotypes of V. cholerae O1.All were resistant to O/129,strepto- mycin,cotrimoxazole,furazolidone and polymyxin B but sensitive to erythromycin tetracycline chram- phenical,norfloxacin,ciprofloxacin,amplcillin and kanamycin. Although some of the biological proper- ties between isolated 4 strains O139 and O1 waq different,but it is not enough to afford evidence for dif- ferential diagnosis.Further studies on more isolated strains are needed.