间接免疫金银染色检测血清抗核抗体

应用间接免疫金银染色（ⅡGSS）检测了系统性红斑狼疮等自身免疫性疾病病人及正常人血清中的抗核抗体（ANA），并与间接荧光抗体试验（IFAT）作了比较，在149份血清中ⅡGSS检出45份阳性，IFAT只检出29份阳性。IFAT有32份为可疑，ⅡGSS只有2份为可疑，且ANA滴度以ⅡGSS为高，核型显示以ⅡGSS更清楚，结果表明，ⅡGSS检测血清ANA特异性高，较IFAT更敏感、可靠、并能反映ANA组分。

APPLICATION OF INDIRECT IMMUNOGCLD-SLVER STAINING IN DETECTING SERAL ANTINUCLEAR ANTIBODIES

Indirect immunogold-silver staining ( IIGSS ) was applied to detect anti-nuclear antibodies ( ANA )in sera from 115 patients with autoimmune diseases, such as systemic lupus erythematosus, etc, and from 34 normal persons. The results were compared with those obtained simultaneously from indirect fluoresent antibody test ( IFAT ) . Forty-five of the 1^9 sera were found to be ANA positive by IIGSS, but 29 by IFAT. And doubtful results appeared in IFAT. However,2 doubtful results in IIGSS Further, the ANA titer for IIGSS was higher and the patterns of nuclear staining were clearer than those in IFAT. These results indicate that IIGSS. is highly specific, sensitive and reliable in detecting serai ANA. The patterns of nuclear staining with IIGSS are also mentioned in this paper.