高效抗逆转录病毒治疗对T细胞亚群上第二受体影响的研究

目的；研究高效抗逆转录病毒治疗(HAART)后T细胞亚群上第二受体的变化及其意义。方法：采用流式细胞仪检测CD4^ 、CD8^-、CD4^ CD45RA^ 以及CD4^ CD45RO^ 细胞表面CCR5及CXCR4的表达水平。结果：HIV感染后，第二受体在CD4和CD8以及记忆型和处女型CD4T细胞亚群上的表达水平，与正常对照没有显著性差异。HAART治疗仪能在一定程度上改变细胞表面的第二受体水平。结论：第二受体参与了HIV的致病过程，B期之前开始抗病毒治疗将有较好的疗效。

Expression of co-receptor on different T lymphocytes subpopulations after effective HAART

Objective: To measure CCR5 and CXCR4 chemokine receptor expression on CD4 and CD8 T cells in HIV 1 infection and to relate levels to the distribution of CD45RO memory and CD45RA naive subsets after effective HAART. Methods: Four color cytofluorometry with appropriate conjugated monoclonal antibodies (mAbs) was performed to define CD45RA and CD45RO subsets of CD4 and CD8 T cells and measure the expression of CCR5, CXCR4 in blood from 43 received HAART patients and 5 non treated HIV and 13 healthy controls. Results: The levels of CCR5 and CXCR4 on CD4 and CD8 T cells and their CD45RO/CD45RA subsets in HIV 1 infected patients had not any statistical significance than that on control subjects and effective HAART could adjust the expression on T cells. Conclusion: CXCR4/CCR5 plays an important role in the progress of HIV 1 infection. The most favorable condition for treatment should be initiated before stage B.