| Relationship between the Expression of RASSF1A Protein and Promoter Hypermethylation of RASSF1A Gene in Bladder Tumor |
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| 作者姓名： | 胡建庭 李宏召 史涛坪 马鑫 王保军 徐华 艾星 居正华 王超 张国玺 张旭 |
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| 作者单位： | Department of Urology Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China，Department of Urology，Tongji Hospital，Tongji Medical College，Huazhong University of Science and Technology Wuhan 430030 China |
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| 摘 要： | To investigate the relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene, RASSF1A protein expression was measured by Western blotting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carcinoma （BTCC）. The promoter methylation in BTCC and normal bladder tissues was detected by methylation-specific PCR （MSP）. The results showed that the expression level of RASSF1A protein was significantly lower in BTCC tissues than that in normal bladder tissues. However, it was not correlated with its clinical stages and pathological grades. The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues. In 14 patients with the aberrant promoter methylation, 13 showed loss or low expression of RASSF 1A protein. It is concluded that RASSF1A gene promoter methylation may contribute to the low level or loss of RASSF1A protein expression, the inactivation of RASSF1A gene and the genesis of BTCC. But, it may bear no correlation with its clinical stages and pathological grades.
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| 关 键 词： | 膀胱肿瘤 蛋白质 基因 启动子 治疗方法 |
Relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene in bladder tumor |
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| Jianting Hu,Hongzhao Li,Taoping Shi,Xin Ma,Baojun Wang,Hua Xu,Xiang Ai,Zhenghua Ju,Chao Wang,Guoxi Zhang,Xu Zhang.Relationship between the Expression of RASSF1A Protein and Promoter Hypermethylation of RASSF1A Gene in Bladder Tumor[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2008,28(2):182-184. |
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| Authors: | Jianting Hu Hongzhao Li Taoping Shi Xin Ma Baojun Wang Hua Xu Xiang Ai Zhenghua Ju Chao Wang Guoxi Zhang Xu Zhang |
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| Institution: | Department of Urology, Tong]i Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
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| Abstract: | To investigate the relationship between the expression of RASSF1A protein and promoter hypermethylation of RASSF1A gene,RASSF1A protein expression was measured by Western blot-ting in 10 specimens of normal bladder tissues and 23 specimens of bladder transitional cell carci-noma(BTCC).The promoter methylation in BTCC and normal bladder tissues was detected by me-thylation-specific PCR(MSP).The results showed that the expression level of RASSF1A protein was significantly lower in BTCC tissues than that in normal bladder tissues.However,it was not corre-lated with its clinical stages and pathological grades.The frequency of promoter methylation of RASSF1A gene was higher in BTCC tissues than that in normal bladder tissues.In 14 patients with the aberrant promoter methylation,13 showed loss or low expression of RASSF1A protein.It is con-cluded that RASSF1A gene promoter methylation may contribute to the low level or loss of RASSF1A protein expression,the inactivation of RASSF1A gene and the genesis of BTCC.But,it may bear no correlation with its clinical stages and pathological grades. |
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| Keywords: | bladder transitional cell carcinoma DNA methylation Western-blotting RASSF1A gene |
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