P27^kip1与细胞周期蛋白G在急性白血病中的表达及相关性研究

为了探讨P27^kip1与细胞周期蛋白G（cyclin G）在急性白血病患者中的表达及其相关性，采用逆转录聚合酶链反应（RT—PCR）检测89例急性白血病患者和10例正常人白细胞的P27^kip1与cyclin G mRNA水平，采用Wes—tern blot检测39例急性白血病患者和10例正常人白细胞的P27^kip1与cyclinG蛋白表达水平。结果显示：急性白血病初治／复发组的cyclin G mRNA与蛋白表达水平均明显高于缓解组和正常对照组（P〈0．05或P〈0．01）；缓解组与正常对照组之间无显著差异（P〉0．05）。急性白血病初治／复发组的P27^kip1 mRNA水平与缓解组和正常对照组比较均无明显差异（P〉0．05）。而急性白血病缓解组和正常对照组的P27^kip1的蛋白表达水平高于初治／复发组（P〈0．05），缓解组与正常对照组之间无显著差异（P〉0．05）。P27却。与cyclin G的表达呈低度负相关。结论：P27螂。与cyclin G在白血病患者中有异常表达，与白血病的发生发展有一定联系；P27^kip1的低表达与cyclin G的异常高表达可能是白血病预后不良的因素之一。

Expression of P27kip1 and Cyclin G in Patients with Acute Leukemia and Its Correlation

This study was purposed to explore the expression of P27^kip1 and cyclin G in patients with acute leukemia （AL） and its correlation. The reverse polymerase chain reaction（RT-PCR） was used to analyse the expression of P27^kip1 and cyclin G mRNA in 89 AL patients and 10 normal persons; Western blot was used to analyze the expression of P27^kip1 and cyclin G protein in 39 AL patients and 10 normal persons. The results showed that the cyclin G mRNA and protein expressions in new diagnosed/relapsed cases of AL were significantly higher than those in patients with remission and normal controls （p 〈0.05 and p 〈0.01 ）, but there was no difference between remission cases and normal controls（p 〉 0.05 ）. The expression of P27^kip1 mRNA in newly diagnosed/relapsed patients with AL was not significantly different from patients with remission and normal controls （p 〉 0.05 ）, while the P27^kip1 protein expression in remission cases of AL and normal controls was significantly higher than that in new diagnosed/relapsed cases（p 〈 0.05 ）, but there was no difference between remission cases and normal controls （p 〉 0.05 ）. The expression of P27^kip1 negatively and lowly correlated with the expression of cyclin G in patients with AL. It is concluded that the low expression of P27^kip1 and the high expression of cyclin G in patients with AL may have some correlation with genesis and development of AL and may be an indication for poor prognosis of AL.