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| P27^kip1与细胞周期蛋白G在急性白血病中的表达及相关性研究 | |
| 引用本文： | 陈惠瑜,林东红,罗玲清,胡建达.P27^kip1与细胞周期蛋白G在急性白血病中的表达及相关性研究[J].中国实验血液学杂志,2009,17(4):847-851. |
| 作者姓名： | 陈惠瑜 林东红 罗玲清 胡建达 |
| 作者单位： | 1. 福建省妇幼保健院检验科,福建福州,350001 2. 福建医科大学检验系,福建福州,350004 3. 福建省肿瘤医院检验科,福建福州,350014 4. 福建医科大学协和医院血液病研究所,福建福州,350001 |
| 基金项目： | 福建省自然科基金资助项目，福建省科技厅三项费基金资助项目 |
| 摘 要： | 为了探讨P27^kip1与细胞周期蛋白G（cyclin G）在急性白血病患者中的表达及其相关性，采用逆转录聚合酶链反应（RT—PCR）检测89例急性白血病患者和10例正常人白细胞的P27^kip1与cyclin G mRNA水平，采用Wes—tern blot检测39例急性白血病患者和10例正常人白细胞的P27^kip1与cyclinG蛋白表达水平。结果显示：急性白血病初治／复发组的cyclin G mRNA与蛋白表达水平均明显高于缓解组和正常对照组（P〈0．05或P〈0．01）；缓解组与正常对照组之间无显著差异（P〉0．05）。急性白血病初治／复发组的P27^kip1 mRNA水平与缓解组和正常对照组比较均无明显差异（P〉0．05）。而急性白血病缓解组和正常对照组的P27^kip1的蛋白表达水平高于初治／复发组（P〈0．05），缓解组与正常对照组之间无显著差异（P〉0．05）。P27却。与cyclin G的表达呈低度负相关。结论：P27螂。与cyclin G在白血病患者中有异常表达，与白血病的发生发展有一定联系；P27^kip1的低表达与cyclin G的异常高表达可能是白血病预后不良的因素之一。 |
| 关 键 词： | P27^kip1 细胞周期蛋白G 急性白血病 |
Expression of P27kip1 and Cyclin G in Patients with Acute Leukemia and Its Correlation |
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| CHEN Hui-Yu,LIN Dong-Hong,LUO Ling-Qing,HU Jian-Da.Expression of P27kip1 and Cyclin G in Patients with Acute Leukemia and Its Correlation[J].Journal of Experimental Hematology,2009,17(4):847-851. | |
| Authors: | CHEN Hui-Yu LIN Dong-Hong LUO Ling-Qing HU Jian-Da |
| Institution: | ( Department of Laboratory Examination, Fujian Provincial Maternal and Children Hospital, Fuzhou 350001, Fujian Province, China; 1 Department of Medical Laboratory Examination, Fujian Medical University, Fuzhou 350004, Fujian Province, China; z Fujian Province Tumour Hospital, Fuzhou 350014, Fujian Province, China; 3 Fujian Institute of Hematology, The Union Hospital, Fujian Medical University, Fuzhou 350001 ,Fujian Province, China) |
| Abstract: | This study was purposed to explore the expression of P27^kip1 and cyclin G in patients with acute leukemia （AL） and its correlation. The reverse polymerase chain reaction（RT-PCR） was used to analyse the expression of P27^kip1 and cyclin G mRNA in 89 AL patients and 10 normal persons; Western blot was used to analyze the expression of P27^kip1 and cyclin G protein in 39 AL patients and 10 normal persons. The results showed that the cyclin G mRNA and protein expressions in new diagnosed/relapsed cases of AL were significantly higher than those in patients with remission and normal controls （p 〈0.05 and p 〈0.01 ）, but there was no difference between remission cases and normal controls（p 〉 0.05 ）. The expression of P27^kip1 mRNA in newly diagnosed/relapsed patients with AL was not significantly different from patients with remission and normal controls （p 〉 0.05 ）, while the P27^kip1 protein expression in remission cases of AL and normal controls was significantly higher than that in new diagnosed/relapsed cases（p 〈 0.05 ）, but there was no difference between remission cases and normal controls （p 〉 0.05 ）. The expression of P27^kip1 negatively and lowly correlated with the expression of cyclin G in patients with AL. It is concluded that the low expression of P27^kip1 and the high expression of cyclin G in patients with AL may have some correlation with genesis and development of AL and may be an indication for poor prognosis of AL. |
| Keywords: | P27kip1 |
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