| RP-HPLC法测定杜仲补天素片中芍药苷的含量 |
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| 引用本文： | 李惠珍,罗君,周兰,赵琳珺.RP-HPLC法测定杜仲补天素片中芍药苷的含量[J].中国民族民间医药杂志,2011,20(7):45-46. |
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| 作者姓名： | 李惠珍 罗君 周兰 赵琳珺 |
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| 作者单位： | 李惠珍,罗君,Li Hui-Zhen,Luo Jun(贵阳中医学院第一附属医院,贵州,贵阳,550001);周兰,Zhou Lan(贵州省药品检验所,贵州,贵阳,550004);赵琳珺
,Zhao Lin-Jun(贵阳新天卫生服务中心,贵州,贵阳,550002) |
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| 摘 要： | 目的：建立杜仲补天素片中芍药苷的含量测定方法。方法：采用HypersilODS色谱柱（4.6 mm×250mm,5μm）,甲醇-0.1%磷酸（11：89）为流动相;检测波长为230nm,流速为1.0mL.min-1,柱温为30℃。结果：芍药苷在0.02352~0.21168μg进样量与峰面积呈良好的线性关系（r=1.0000）,平均加样回收率（n=6）为98.06%（RSD=1.43%）。结论：本方法操作简便、灵敏、准确,可用于杜仲补天素片的质量控制。
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| 关 键 词： | 杜仲补天素片 芍药苷 RP-HPLC 含量测定 |
Determination of Paeoniflorin in Eucommia Bark Butiansu tablet by RP-HPLC |
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| Li Hui-Zhen,Luo Jun,Zhou Lan,Zhao Lin-Jun.Determination of Paeoniflorin in Eucommia Bark Butiansu tablet by RP-HPLC[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2011,20(7):45-46. |
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| Authors: | Li Hui-Zhen Luo Jun Zhou Lan Zhao Lin-Jun |
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| Institution: | Li Hui-Zhen1,Luo Jun1,Zhou Lan2,Zhao Lin-Jun3 1.The first affiliated hospital of Guiyang College of Traditional Chinese Medicine,Guiyang 550001,China,2.Institute for Drug Control of GuiZhou Province,GuiZhou 550004,3.The Community Health Service of Guiyang xintian |
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| Abstract: | Objective： To develop a method for the determination of Paeoniflorin in Eucommia Bark Butiansu tablet. Methods： Hypersil ODS （4. 6mm× 250mm, 5μm） was used, the mobile phase was methanol - water containing 0. 1% phosphoric acid （ 11 ： 89） at a flow rate of 1. 0mL · min^-1 . The UV detection wavelength was at 230 nm, and the column temperature was at 30℃ . Result： The linear range was 0. 02352 - 0. 21168μg （ r = 1. 0000） for Paeoniflorin. The average recovery （ n = 6） was 98.06% （ RSD = 1.43% ） . Conclusion： The method is found to be simple and accurate for analysis of Paeoniflorin in Eucommia Bark Butiansu tablet. |
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| Keywords: | RP-HPLC |
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