| 中药独活中总香豆素高通量分析方法的建立 |
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| 引用本文： | 李晋,郭新荣,张秋红,常艳旭.中药独活中总香豆素高通量分析方法的建立[J].中国民族民间医药杂志,2011,20(9):48-49. |
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| 作者姓名： | 李晋 郭新荣 张秋红 常艳旭 |
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| 作者单位： | 天津市现代中药重点实验室天津中医药大学,天津,300193 |
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| 摘 要： | 建立中药独活中总香豆素的含量测定的高通量的分析方法。方法：采用紫外分光光度法,检测波长为320nm对中药独活药材中的总香豆素进行测定。结果：采用该方法可准确测定中药独活药材中总香豆素的含量,蛇床子素在3.0-60μg/mL范围内,吸光度与浓度的线性关系良好（r=0.9995）,样品平均回收率为98.1%,精密度RSD为0.27%,稳定性RSD为0.9%（24h）,重复性RSD为1.83%。结论：该方法简单、准确,可用于中药独活药材中总香豆素的含量测定。
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| 关 键 词： | 独活 总香豆素 紫外分光光度法 含量测定 |
Determination of Total Coumarins in Radix Angelicae Pubescentis |
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| Li Jin,Guo Xin-rong,Zhang Qiu-hong,Chang Yan-xu.Determination of Total Coumarins in Radix Angelicae Pubescentis[J].Chinese Journal of Ethnomedicine and Ethnopharmacy,2011,20(9):48-49. |
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| Authors: | Li Jin Guo Xin-rong Zhang Qiu-hong Chang Yan-xu |
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| Institution: | Li Jin,Guo Xin-rong,Zhang Qiu-hong,Chang Yan-xu Tianjin State Key Laboratory of Modern Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China |
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| Abstract: | An UV spectrophotometry for determining total coumarins in Radix Angelicae Pubescentis is established.The powder of Radix Angelicae Pubescentis is extracted by ultrasonic technology.The total coumar ins is detected at 320nm by UV spectrophotometry.T here is a good linear relationship within the range of 3-60 ug/ mL(r = 0.9991).The relative standard deviation of accurate test and stability test are 0.17% and 0.37% respectively.The average recovery is 98.5%(n= 6) with RSD of 0.55%.The method issimple and accu... |
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| Keywords: | Radix Angelicae Pubescentis total coumarins UV spectrophotometry content determination |
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