黄芪注射液对脂多糖诱导的内皮细胞炎症因子产生的影响及其机制研究

目的:观察黄芪注射液对脂多糖(LPS)诱导的内皮细胞炎症因子产生的影响,并初步探讨黄芪注射液的抗炎作用机制。方法:体外培养的人脐静脉内皮细胞(HUVECs),待细胞生长到融合状态时加入不同浓度(10、20、40 mg/L)的黄芪注射液预处理2 h,然后加入LPS(1 mg/L)作用24 h。用四唑盐比色实验(MTT)检测细胞活力;半定量逆转录-聚合酶链式反应(RT-PCR)和酶联免疫吸附法(ELISA)分别检测内皮细胞细胞间黏附分子-1(ICAM-1)、白介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)mRNA的表达和其在上清液中的含量;实时荧光定量PCR(real-time PCR)检测Toll样受体4(TLR4)mRNA的表达;凝胶电泳迁移率实验(EMSA)检测NF-κB活性。结果:LPS能明显降低细胞活力,并能显著增加ICAM-1I、L-8和TNF-αmRNA的表达和含量,同时TLR4 mRNA的表达和NF-κB的活性也显著升高,而预先应用不同浓度的黄芪注射液处理后,上述效应明显减弱。结论:黄芪注射液抑制炎症因子的产生可能与其抑制TLR-4/NF-κB信号通路有关。

Effect and mechanism of radix astragali injection on production of inflammatory factors induced by lipopolysaccharide in human umbilical vein endothelial cells

AIM:To observe the effect of radix astragali injection on the production of inflammatory factors induced by lipopolysaccharide(LPS) and investigate the anti-inflammation mechanism of radix astragali injection. METHODS: The human umbilical vein endothelial cells(HUVECs) were treated with LPS(1 mg/L) for 24 h following pretreatment with various concentrations of radix astragali injection(10,20 or 40 mg/L) for 2 h.Cell viability was detected by MTT;The mRNA expression and levels of intercellular adhesion molec...