| 百里醌抑制大肠癌生长的实验研究 |
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| 引用本文： | 吴玉海,谢丽微.百里醌抑制大肠癌生长的实验研究[J].中国临床药理学与治疗学,2011,16(6):632-636. |
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| 作者姓名： | 吴玉海 谢丽微 |
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| 作者单位： | 1. 浙江省平阳县人民医院普外科,平阳325400,浙江
2. 温州医学院附属第二医院病理科,温州325027,浙江 |
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| 摘 要： | 目的:探讨百里醌抑制体内外大肠癌生长的影响及机制。方法:不同浓度百里醌作用人大肠癌细胞株SW480后,CCK-8法检测细胞增殖;流式细胞术检测细胞凋亡;Western blotting检测大肠癌细胞中NF-κB、Bcl-2和Survivin的表达;建立裸鼠大肠癌皮下移植瘤模型,随机分为对照组和实验组(n=10),第3周开始分别经灌胃给予溶媒(1%乙醇)和百里醌(3 mg/只),每周3次,共两周,术后第8周处死裸鼠,测量肿瘤瘤重并计算抑瘤率;免疫组织化学法检测肿瘤组织的NF-κB、Bcl-2和Survivin的表达。结果:与对照组相比,百里醌可显著抑制大肠癌SW480细胞生长,并诱导细胞凋亡;百里醌可明显抑制NF-κB、Bcl-2和Survivin在SW480细胞中表达;与对照组相比较,实验组裸鼠皮下移植瘤生长被显著抑制,肿瘤组织中NF-κB、Bcl-2和Survivin表达下调。结论:百里醌具有抑制体内外大肠癌生长的作用,可能是通过抑制大肠癌中NF-κB及其调控蛋白Bcl-2及Survivin的表达而实现。
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| 关 键 词： | 百里醌 大肠癌 NF-κB |
An experimental study on inhibition effect of thymoquinone on human colon cancer |
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| WU Yu-hai,XIE Li-wei.An experimental study on inhibition effect of thymoquinone on human colon cancer[J].Chinese Journal of Clinical Pharmacology and Therapeutics,2011,16(6):632-636. |
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| Authors: | WU Yu-hai XIE Li-wei |
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| Institution: | WU Yu-hai2,XIE Li-wei11Department of Pathology,Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,Zhejiang,China,2Department of Surgery,County People's Hospital of Pingyang,Pingyang 325400 |
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| Abstract: | AIM:To investigate the effect and the mechanism of thymoquinone in the growth inhibition of human colon cancer in vitro and in vivo. METHODS: After human colon cancer SW480 cells were treated with different concentrations of thymoquinone,the cellular proliferation was detected by Cell Counting Kit-8(CCK-8) assay.The flow cytometry(FCM) was used to determine apoptosis in SW480 cells.Western blotting was used to detect the protein expression of NF-B,Bcl-2 and Survivin.SW480 cells were injected subcutaneously ... |
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| Keywords: | Thymoquinone Colon cancer NF-B |
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