Complete genome sequence of a dahlia common mosaic virus isolate from New Zealand |
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Authors: | Hadfield James Linderme Daphné Shepherd Dionne N Bezuidenhout Marion Lefeuvre Pierre Martin Darren P Varsani Arvind |
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Affiliation: | (1) School of Biological Sciences, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand;(2) Electron Microscope Unit, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa;(3) Department of Molecular and Cell Biology, University of Cape Town, Rondebosch, Cape Town, 7701, South Africa;(4) Computational Biology Group, Institute of Infectious Diseases and Molecular Medicine, University of Cape Town, Observatory, Cape Town, 7925, South Africa;(5) CIRAD, UMR 53 PVBMT CIRAD-Universit? de la R?union, P?le de Protection des Plantes, Ligne Paradis, Saint Pierre, 97410, La R?union, France;(6) Biomolecular Interaction Centre, University of Canterbury, Private Bag 4800, Christchurch, 8140, New Zealand; |
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Abstract: | Dahlia mosaic disease of the ornamental flowering plant Dahlia is caused by two caulimoviruses, dahlia mosaic virus (DMV) and dahlia common mosaic virus (DCMV). We used a rolling-circle amplification method to amplify, clone and determine for the first time the full genome sequence of a DCMV isolate from New Zealand (DCMV-NZ). Within the 7949-bp circular double-stranded retro-transcribing DCMV-NZ DNA, we identified six putative open reading frames, typical of all genomes in the family Caulimoviridae. The availability of the complete DCMV sequence provides a reference genome against which all others can be compared. |
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