妊娠期高血压疾病与血管紧张素转换酶基因多态性的关系

目的:探讨妊娠期高血压疾病(pregnancy induced hypertension,PIH)与血管紧张素转换酶基因多态性的关系.方法:运用多聚酶链反应技术检测54例妊娠期高血压疾病患者,100例正常妊娠妇女血管紧张素转换酶基因多态.结果:妊娠期高血压疾病患者ACE基因II、ID、DD基因型频率分别为29.6%、25.9%、44.5%,对照组II、ID、DD基因型频率分别为42.0%、39.0%、19.0%,两组D等位基因各为57.4%、38.5%.两组的DD基因型及D等位基因频率比较差异有显著性意义.结论:ACE基因多态性与妊娠期高血压疾病的发病有关.

Study on A Deletion Polymorphism of the Angiotensin Converting Enzyme Gene in Pregnancy Induced Hypertension

Objective:To study on a polymorphism and allele frequency of the angiotensin converting enzyme(ACE) gene in pregnancy induced hypertension.Methods: Polymerase chain reaction(PCR) for detection of ACE gene polymorphism was performed to show the deletion/insertion(D/I) polymorphism in intron 16 of ACE gene.490bp(I) and 190bp(D) PCR products were identified,corresponding to the PCR amplification of the allele with or without the insertion.Results: Derived allele frequencies for insertion and deletion were different between pregnancy induced hypertension patients and control subjects.The frequency of D allele gene and percent of ACE DD genotype were higher in pregnancy induced hypertension(0.574 and 44.5%,respectively) compared with the controls(0.385 and 19.0%)(P<0.05).Conclusion: There was an excess of DD genotype and the frequency of D allele gene in pregnancy induced hypertension,indicating that genetic variation in the ACE gene could be involved in the risk of patients and the ACE gene may contribute to the pathogenesis of pregnancy induced hypertension.