| 银屑病患者皮损p16INK4a基因CpG甲基化位点和频度 |
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| 引用本文： | 陈敏,崔盘根,姚煦,曹元华,弓娟琴,李安生,陈志强.银屑病患者皮损p16INK4a基因CpG甲基化位点和频度[J].中华医学遗传学杂志,2007,24(6):674-676. |
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| 作者姓名： | 陈敏 崔盘根 姚煦 曹元华 弓娟琴 李安生 陈志强 |
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| 作者单位： | 1. 中国医学科学院,中国协和医科大学皮肤病研究所皮肤病研究室,南京,210042
2. 中国医学科学院,中国协和医科大学皮肤病研究所检验科,南京,210042 |
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| 基金项目： | 江苏省人事厅“六大人才高峰”项目，国家自然科学基金(30170863)~~ |
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| 摘 要： | 目的研究皮损表皮p16INK4a甲基化阳性的银屑病患者基因启动子区CpG甲基化的位点和频度。方法采集50例银屑病患者皮损处皮肤,分离表皮,提取DNA。采用甲基化特异性PCR和测序法检测p16INK4a基因启动子区CpG甲基化的位点和频度。结果p16INK4a基因启动子甲基化阳性的患者,约50%CpG发生甲基化,均出现于特定位点。结论p16INK4a甲基化阳性银屑病患者p16INK4a基因启动子并非完全发生甲基化。
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| 关 键 词： | 银屑病 p16~(INK4a)基因 CpG甲基化 启动子 |
| 收稿时间： | 2007-01-20 |
The methylation locus and frequency pattern on p16INK4a gene promoter CpG in epidermis of patients with psoriasis |
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| CHEN Min,CUI Pan-gen,YAO Xu,CAO Yuan-hua,GONG Juan-qin,LI An-sheng,CHEN Zhi-qiang.The methylation locus and frequency pattern on p16INK4a gene promoter CpG in epidermis of patients with psoriasis[J].Chinese Journal of Medical Genetics,2007,24(6):674-676. |
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| Authors: | CHEN Min CUI Pan-gen YAO Xu CAO Yuan-hua GONG Juan-qin LI An-sheng CHEN Zhi-qiang |
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| Institution: | Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, Jiangsu, 210042 PR China. |
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| Abstract: | Objective To investigate the CpG methylation locus and frequency pattern on p16INK4a gene promoter in epidermis of p16INK4a methylated patients with psoriasis vulgaris.Methods The DNA specimens were obtained from epidermal lesion of 50 plaque psoriatic patients.Methylation specific PCR and DNA sequencing were used to detect the frequency and locus of methylation in p16INK4a gene promoter region.Results Approximately 50% CpG was methylated in p16INK4a methylated patients,methylation was found in specifical locus of p16INK4a gene promoter.Conclusion The distinct methylation pattern is showed on the p16INK4a gene promoter region in patients with psoriasis. |
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| Keywords: | psoriasis p16~(INK4a) gene CpG methylation promoter |
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