WUT6-抗人胸腺细胞共同性分化抗原来交瘤细胞系的建立

本文报导了用人胎儿胸腺细胞免疫BALB/c小鼠,取其脾细胞与小鼠骨髓瘤细胞系NS-1融合,获得一株能稳定分泌抗人共同性胸腺细胞单克隆抗体的杂交瘤细胞株,W_(?)T_(?)。它与72％胸腺细胞、皮肤Langerhan's细胞及部分T细胞系呈阳性反应;外周血T、D细胞、粒细胞、红细胞、血小板及B细胞系、Null细胞系均阴性。FACS检测结果及W_(?)T_(?)阳性细胞的组织分布特点均同HIT_6相似。在胸腺、皮质细胞及髓质中少数胞体较大呈树突状的细胞呈阳性反应。在扁桃体实质中少数散在细胞阳性。上皮基底层中的Langerhan's细胞则呈强阳性反应。用W_(?)T_(?)亲和层析柱提取胸腺细胞膜上相应的靶抗原分子,证明W_(?)T_(?)识别分子量为51KDa的抗原。免疫竞争抑制试验表明,W_(?)T_(?)同抗原的结合可被HIT_6完全阻断。说明W_(?)T_(?)与HIT_6识别相同的抗原决定簇。

Establishment of Hybridoma Secreting McAb (WuT6) Against Human Commom Thymocyte Differentiation Antigen

A mouse McAb(WuT6) against human common thymocyte differentiation antigen from hybridoma fused between Immunized BALB/c spleen cells and NS-1 myeloma cells was obtained. The McAb reacted with 72% thymocytes, Langerhan's cells and some of T cell lines, but rarely with peripheral blood mononuclear cells. The histological distribution of WuTe positive cells in thymus and tonsil was similar to HIT6-1, 2(CDla). The competitive blocking test showed that the combining of FITC labeled WuTe with its target antgien could be blocked completely by unlabeled WuTe and HIT6-1, 2, sug-. gesting these two McAbs recognizing a same epitope on thymocytes. Using WuT6 affinity chromatographic column to isolate the membrane antigen of thymocytes and then SDS-PAGE, the molecular weight of the WuT6 recognized antigen was 5lKDa. WuTe was OKTe analogue recognizing human thymocyte differentiation antigen and Langerhan's cells.