血小板衍生内皮细胞生长因子在胃癌和胃癌前病变中的表达及意义

目的：分析胃癌和胃癌前病变组织中血小板衍生内皮细胞生长因子（thymidine phosphorylase/platelet-derived endothelial cell growth factor, TP/PD-ECGF）表达、微血管密度（microvessel density MVD）和血小板（PLT）计数的变化.方法：收集有详细临床资料,并有外周血血小板计数资料的归档病理切片标本117例,分别作TP/PD-ECGF、MVD（应用CD34染色）免疫组化染色和PLT计数.结果：正常胃黏膜、肠上皮化生、不典型增生和肠型胃癌组织中TP/PD-ECGF表达阳性率分别为25%、34.88%、65.71%和92.95%,各组间差异有统计学意义.117例不同胃组织中TP/PD-ECGF表达积分与MVD具有相关性（r=0.441,P=0.000）,TP/PD-ECGF表达阳性组与阴性组的MVD差异有统计学意义,P＜0.01.胃不典型增生组织TP/PD-ECGF表达阳性组与阴性组的MVD差异有统计学意义,P＜0.05.对MVD行4组不同胃组织间的两两比较显示,除肠上皮化生组与不典型增生组之间差异无统计学意义外（P＞0.05）,其余组间比较均有统计学意义.血小板计数在正常胃黏膜组与肠型胃癌组之间差异有统计学意义,P＜0.01.结论：胃癌和胃癌前病变中存在TP/PD-ECGF表达增加和MVD增多.伴随TP/PD-ECGF表达增加,MVD也随之增加.血小板在胃癌的形成中也起到一定作用.

Expression of thymidine phosphorylase/platelet-derived endothelial cell growth factor in gastric carcinoma and precancerous lesions

OBJECTIVE:To find the difference of TP/PD-ECGF, MVD and platelet count between gastric carcinoma and precancerous lesions. METHODS: A total of cases of pathological slices of gastric tissue specimens and corresponding platelet count data were obtained. Mouse anti-human TP/PDECGF monoclonal antibody and mouse anti-human CD34 monoclonal antibody were used in the immunohistochemistry method. RESULTS: The positive ratios of TP/PD-ECGF in normal mucosa of stomach, intestinal metaplasia, dysplasia and intestinal-type gastric carcinoma were 25%, 34.88%, 65.71% and 92.95% respectively. The expressions of TP/PD-ECGF were significantly different in various groups. MVD was correlated with the score of expression of TP/PD-ECGF in all 117 cases (r=0.441, P=0.000). MVD was significantly different between the TP/PD-ECGF positive and negative groups of all 117 cases (P<0.01). The MVD in dysplasia group was also significantly different between the TP/PD-ECGF positive and negative groups (P<0.05). Platelet count was significantly differenbetween the normal gastric tissue group and gastric carcinoma group. CONCLUSION: As a result there is more TP/PD-ECGF expression and MVD in gastric carcinoma and precancerous lesions. The MVD is increased with the more expression of TP/PD-ECGF. It is suggested that the platelet may contribute to gastric carcinoma progression.