脐带间充质干细胞对脑梗死大鼠行为学及胆碱能的影响

目的：观察脑梗死大鼠经人脐带间充质干细胞（umbilical cord-derived mesenchymal stem cells，UC-MSCs）治疗后，行为学的恢复情况，2，3，5-氯化三苯基四氮唑（triphenyl tetrazolium chloride，TTC）染色以及胆碱能的变化。方法制备脑梗死大鼠大脑中动脉闭塞（Middle cerebral artery occlusion，MCAO）模型，将 UC-MSCs 注入脑梗死大鼠体内，对大鼠运动功能进行评分，比较 TTC 染色显示的脑梗死区域所占比例，测定脑组织和血浆中乙酰胆碱酯酶（True choline esterase，TChE）活性和乙酰胆碱（Acetylcholine，Ach）浓度。结果与模型组相比，UC-MSCs 组大鼠行为学恢复明显（P ＜0．05），UC-MSCs 组大鼠脑组织和血浆乙酰胆碱酯酶（TChE）和乙酰胆碱（Ach）活性显著增强（P＜0．05）。结论UC-MSCs 经尾静脉移植可促进脑梗死后神经功能恢复，增强大鼠胆碱能系统活性。

Effects of ethology,TChE and Ach activities in rat model of Middle cerebral artery occlusion administrated by human um- bilical cord-derived Mesenchymal stem cells

Objective To observe the effects of intravenous administration of human umbilical cord-derived mesen-chymal stem cells（UC-MSCs）after middle cerebral artery occlusion（MCAO）in adult rats.Methods Adult male SD rats were administrated by MCAO.Functional outcome measurements using Beam walking Test（BWT）were performed at 24 hour of MCAO group and 10 days of UC-MSCs treatment group respectively.TTC staining ,TChE and Ach activity array were also used.Results There was significant neurological function improvement in rats treated with MSC com-paring with that of control groups（P 〈0.05）,and also Ach and TChE activity （P 〈0.05）.Conclusion Intravenous ad-ministration of UC-MSCs promotes the neurological function in rat MCAO model.UC-MSCs play an advantageous function on cholinergic neurotransmitters in the case of lesion.