咖啡因增强UVB辐射诱导的HaCaT细胞凋亡的研究

目的：观察咖啡因对中波紫外线（Ultraviolet B,UVB）辐射诱导的HaCaT细胞凋亡的影响。方法：在DMEM中培养HaCaT细胞,将细胞分成空白对照组（A组）,1mM咖啡因处理组（B组）,5mM咖啡因处理组（C组）,UVB（20mJ/cm2）辐射组（D组）,UVB辐射（20mJ/cm2）＋1mM咖啡因处理组（E组）,UVB辐射（20mJ/cm2）＋5mM咖啡因处理组（F组）,Annexi n V/PI观察各组细胞凋亡比例,Westernblot检测各组细胞p21和BCL-2表达情况。结果：20mJ/cm2UVB能诱导HaCaT细胞凋亡增加（A组凋亡率5.43%,D组凋亡率20.67%）,而1mM和5mM咖啡因能进一步促进HaCaT细胞凋亡（E组凋亡率22.25%,F组凋亡率36.61%）,UVB抑制细胞p21和BCL-2蛋白表达,咖啡因能进一步抑制21和BCL-2蛋白表达。结论：UVB辐射能诱导HaCaT细胞凋亡,抑制p21和BCL-2蛋白表达,咖啡因则能进一步促进凋亡,抑制p21和BCL-2蛋白表达。

The effect of caffeine to UVB irradiation induced cell apoptosis of HaCaT cells

Objective To investigate the effect of caffeine on UVB irradiation induced cell apoptosis of HaCaT cells.Methods HaCaT cells were cultured in DMEM medium.Cells were divided into 6 groups.A group： Blank control（without UVB and caffeine）;B group： 1mM caffeine,without UVB;C group： 5mM caffeine,without UVB;D group： without caffeine,20mJ/cm2 UVB;E group： 1mM caffeine,20mJ/cm2 UVB;F group： 5mM caffeine,20mJ/cm2 UVB.Annexin V/PIKit was used to detect cell apoptosis.Western blot was used to detect the expression of p21 and BCL-2.Results 20mJ/cm2 UVB irradiation could induce cell apoptosis of HaCaT cells（cell apoptosis rate is 5.43% in group A and 20.67% in group D）,1mM and 5mM caffeine could promote cell apoptosis of HaCaT cells further（cell apoptosis rate is 22.25% in group E and 36.61% in group F）.UVB irradiation could inhibite the expression of p21 and BCL-2,caffeine could inhibite p21 and BCL-2 futher.Conclusions UVB irradiation could induce HaCaT cell apoptosis and inhibite the expression of p21 and BCL-2.Caffeine could induce cell apoptosis and inhibite the expression of p21 and BCL-2 further.