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| 西方马脑炎病毒抗体快速检测试纸条的研制 | |
| 引用本文： | 王林林,杨宇,王旺,王振东,王静. 西方马脑炎病毒抗体快速检测试纸条的研制[J]. 中国媒介生物学及控制杂志, 2011, 22(5): 421-423 |
| 作者姓名： | 王林林 杨宇 王旺 王振东 王静 |
| 作者单位： | 1. 中国检验检疫科学研究院,北京100123;沈阳农业大学生物科学技术学院,沈阳110866 2. 中国检验检疫科学研究院,北京,100123 3. 沈阳农业大学生物科学技术学院,沈阳,110866 |
| 基金项目： | 质检公益项目，国家质检总局科技计划项目 |
| 摘 要： | 目的研制一种快速检测西方马脑炎病毒（WEEV）抗体的试纸条。方法利用胶体金标记和免疫层析技术,用25 nm胶体金标记葡萄球菌A蛋白,并用硝酸纤维膜包被WEEV抗原及羊抗兔IgG作为检测带和质控带,研制出WEEV抗体快速检测试纸条。对该试纸条的敏感性、特异性和稳定性做出评价,并拟合检测曲线进行半定量检测。在健康人血清、兔血清和鼠血清中添加WEEV抗体模拟污染样品,评价该方法的检测能力。结果该试纸条可在20 min内完成定性和半定量检测,灵敏度为120 ng/ml,模拟样品的检测灵敏度也相同。线性范围120～1200 ng/ml。对发病症状相似以及近缘的其他病毒进行检测,均无非特异反应。试纸条在37℃下保存1个月,检测结果不变。结论该试纸条具有快速、简便、灵敏、特异、稳定的特点,适用于现场检测。 |
| 关 键 词： | 西方马脑炎病毒 免疫层析技术 胶体金试纸 定性检测 定量检测 |
Design of a test strip for rapid detection of antibodies against Western equine encephalitis virus |
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| WANG Lin-lin,YANG Yu,WANG Wang,WANG Zhen-dong,WANG Jing. Design of a test strip for rapid detection of antibodies against Western equine encephalitis virus[J]. Chinese Journal of Vector Biology and Control, 2011, 22(5): 421-423 | |
| Authors: | WANG Lin-lin YANG Yu WANG Wang WANG Zhen-dong WANG Jing |
| Affiliation: | WANG Lin-lin1,2,YANG Yu1,WANG Wang1,WANG Zhen-dong2,WANG Jing1 1 China Academy of Inspection and Quarantine,Beijing 100123,China,2 Shenyang Agricultural University,Institute of Biological Science and Technology,Shenyang 110866,Liaoning Province |
| Abstract: | Objective To develop a test strip for rapid detection of antibodies against Western equine encephalitis virus(WEEV).Methods Staphylococcal protein A was labeled with 25 nm colloidal gold via immune-chromatography;cellulose nitrate-coated WEEV antigens and goat anti-rabbit antibodies were used as the test zone and the control zone,respectively.The sensitivity,specificity and stability of the test strip were evaluated and semi-quantitative detection was conducted with a curve of best fit.The detection capabil... |
| Keywords: | Western equine encephalitis virus Immunochromatography Immunogold labeling Qualitative detection Quantitative detection |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |