地塞米松对热性惊厥患儿外周血单个核细胞细胞间黏附分子-1及淋巴细胞功能相关抗原-1的干预作用

目的 探讨地塞米松(DXM)对热性惊厥(FS)患儿外周血单个核细胞(PBMC)细胞间黏附分子-1(ICAM-1)及配体淋巴细胞功能相关抗原-1( LFA-1)表达和分泌的影响.方法 FS组患儿16例分为FS对照组和DXM干预组,DXM干预组采用1.0μmol·L-1的DXM进行干预；健康对照组16例,为年龄和性别与FS组患儿相匹配的同期体检健康儿童.常规方法进行FS组和健康对照组的PBMC培养.采用流式细胞仪检测PBMC表面ICAM-1、LFA-1表达水平；采用ELISA法检测培养上清中ICAM-1、LFA-1水平.结果 FS对照组在体外培养的PBMC表面ICAM-1、LFA -1表达水平分别为(19.78±8.93)％和(43.05±15.85)％,明显高于健康对照组(13.88±6.73)％,(30.43±16.25)％] (Pa＜0.05)；DXM干预组的PBMC表面ICAM-1、LFA-1表达水平分别为(17.14±7.98)％和(33.93±19.76)％,与FS对照组比较有下调趋势,但差异无统计学意义(Pa＞0.05).FS对照组PBMC的ICAM-1、LFA-1分泌水平分别为(6.73±3.88)％和(4.84±2.95)％,明显高于健康对照组(4.24±2.42)％,(2.76±1.36)％](Pa＜0.05)；DXM干预组PBMC的ICAM-1、LFA-1分泌水平(4.34±1.82)％,(3.06±2.81)％]明显低于FS对照组(Pa＜0.05).结论 糖皮质激素DXM干预后ICAM-1、LFA-1的表达被抑制,抑制FS患儿ICAM-1、LFA-1的高表达可作为其治疗的新靶点.

Intervention of Dexamethasone on Expression Levels of Intercellular Adhesion Molecule-1 and Lymphocyte Function-Associated Antigen-1 mRNA of Peripheral Blood Mononuclear Cells in Children with Febrile Seizures

Objective To analyze the expressions of peripheral blood mononuclear cells(PBMC) intercellular adhesion molecule-1(ICAM-1) and lymphocyte function-associated antigen-1(LFA-1) immunoreaction in patients following febrile seizures(FS),in order to find out the neuroimmunomodulation mechanisms in the pathogenesis of FS. Methods Sixteen cases of FS were divided into 2 groups randomly:FS control group and dexamethasone(DXM)-treated group.Sixteen cases of healthy children whose age and sex were matched to the pati...