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| 逆转录病毒转染法建立兔VX-2多药耐药株 | |
| 引用本文： | 樊爱琳,刘国鹏,郑善銮,马静,王执民,丁振若,杨麦贵,郝晓柯. 逆转录病毒转染法建立兔VX-2多药耐药株[J]. 医学争鸣, 2003, 24(23): 2129-2131 |
| 作者姓名： | 樊爱琳 刘国鹏 郑善銮 马静 王执民 丁振若 杨麦贵 郝晓柯 |
| 作者单位： | 1. 第四军医大学,西京医院检验科,陕西,西安,710033 2. 第四军医大学,基础部教学实验中心,陕西,西安,710033 3. 第四军医大学,西京医院中医科,陕西,西安,710033 4. 第四军医大学唐都医院介入放射科,陕西,西安,710038 |
| 基金项目： | 陕西省科技攻关项目 (CX99A0 1 6) |
| 摘 要： | 目的：建立VX-2多药耐药(MDR)细胞(VX-2mdrI)。方法：利用逆转录病毒转染法将带有mdrI cDNA全序列的逆转录病毒载体pHaMDR1转入到兔VX-2细胞中，MTT法检测细胞在不同化疗药物作用下的存活率；免疫组化检测细胞的P-糖蛋白(P-gp)表达，RT-PCR检测细胞内mdrI mRNA的表达量．结果：转基因的细胞对吡柔比星、秋水仙素的耐药性分别提高10和25倍，免疫组化见转基因细胞中P-gp表达增加，RT-PCR示细胞内mdrI mRNA的表达量增加。结论：利用逆转录病毒转染法构建了兔VX-2 MDR细胞。 |
| 关 键 词： | VX-2细胞 多药耐药 逆转录病毒 基因转移 |
| 文章编号： | 1000-2790(2003)23-2129-03 |
| 修稿时间： | 2003-05-30 |
Construction of MDR rabbit VX-2 cell line by retrovirus infection |
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| FAN Ai Lin ,LIU Guo Peng ,ZHENG Shan Luan ,MA Jing ,WANG Zhi Min ,DING Zhen Ruo ,YANG Mai Gui ,HAO Xiao Ke. Construction of MDR rabbit VX-2 cell line by retrovirus infection[J]. Negative, 2003, 24(23): 2129-2131 | |
| Authors: | FAN Ai Lin LIU Guo Peng ZHENG Shan Luan MA Jing WANG Zhi Min DING Zhen Ruo YANG Mai Gui HAO Xiao Ke |
| Affiliation: | FAN Ai Lin 1,LIU Guo Peng 2,ZHENG Shan Luan 1,MA Jing 3,WANG Zhi Min 4,DING Zhen Ruo 1,YANG Mai Gui 1,HAO Xiao Ke 1 1Department of Clinical Laboratory,Xijing Hospital,2Center of Basic Medical Teaching Experiment,School of Basic Medicine,3Department of Chinese Traditional Medicine,Xijing Hospital,Fourth Military Medical University,Xi'an 710033,China,4Department of Interventional Radiology,Tangdu Hospital,Fourth Military Medical University,Xi'an 710038,China |
| Abstract: | AIM: To establish a multidrug resistance (MDR) rabbit VX 2 (VX 2 mdr 1) cell line. METHODS: Rabbit VX 2 cells were transfected by mdr 1 retrovirus supernatant. MDR1 of rabbit cells were tested by MTT assay and the expression of P glycoprotein (P gp) on membrane and mdr 1 mRNA in cells was detected by immunocytochemistry and RT PCR. RESULTS: The rabbit VX 2 mdr 1 cells were found to be 9.5 times more resistant to pirarubicin and 25 times to colchicine than VX 2 cells. VX 2 mdr 1 cells expressed higher P gp than VX 2 cells on cell membrane and VX 2 mdr 1 cells showed much higher mdr 1 mRNA level than VX 2 cells. CONCLUSION: A MDR rabbit VX 2 cell line is successfully established by gene transfer. |
| Keywords: | VX 2 cell MDR retrovirus gene transfer |
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