乙醇肝损伤小鼠肝脏的基因表达谱研究

 目的研究乙醇肝损伤小鼠肝组织的基因表达谱,筛选乙醇肝损伤相关基因,并探讨其肝损伤机制。方法①将实验小鼠分为正常对照组与乙醇肝损伤组,分别提取2组小鼠肝脏mRNA,经反转录后分别用Cy3,Cy5荧光标记,获得2组动物来源的cDNA探针;②cDNA探针与联合基因公司BiostarM-141S小鼠基因表达谱芯片杂交,结果由扫描仪扫描并用统计软件进行分析。结果与正常对照组相比,乙醇肝损伤组有117条基因发生差异性表达(0.83%),其中46条基因表达上调,另外71条基因表达下调。结论利用表达谱芯片结合实验动物模型能大规模、高通量地研究乙醇肝损伤相关基因,对进一步阐明乙醇对肝脏的损伤机制具有重要的意义。

Gene expression profile of alcoholic liver-injured mice using Cdna microarray

OBJECTIVE To study the gene expression profile of alcohol liver injuried mice and screen the differentially expressed genes that relate to liver injury formation by alcohol on a large scale using cDNA raicroarrays. METHODS The experimental mice were divided into two groups: one was control group and the other was alcohol-administrated group. The mRNA in the livers of the two groups was extracted respectively and reversely transcribed to cDNA with the incorporation of different fluorescent-labeled dUTP (Cy3-dUTP, Cy5-dUTP) as the hybridization probes. The mixed probes were hybridized to the cDNA microarrays. The fluorescent signal values were acquired by scanner and analyzed by statistical software. RESULTS Among the 14 100 target genes, 117 genes were differentially expressed of in which 46 genes were up-regulated and another 71 genes were down-regulated. They were closely related to a range of biological functions. CONCLUSION Using cDNA microarray and experimental animal modeling technique can study the differentially expressed genes of alcoholic liver injury in mouse on a large scale. It is useful for further investigation of the injury molecular mechanism of alcohol.