基质金属蛋白酶-9及其组织金属蛋白酶抑制剂-1表达与大肠癌侵袭转移的研究

目的:探讨基质金属蛋白酶-9(MMP鄄9)及其相应组织金属蛋白酶抑制剂-1(TIMP鄄1)在大肠癌组织中的表达与大肠癌侵袭转移的关系。方法:应用免疫组织化学SP法检测了48例大肠癌组织标本及23例正常大肠标本MMP鄄9、TIMP鄄1的表达。结果:48例大肠癌组织MMP鄄9阳性率为87.5%,显著高于正常大肠标本(P<0.05),伴有淋巴结转移或肝转移者MMP鄄9的阳性率明显高于无淋巴结转移或肝转移者(P<0.05)。大肠癌组织TIMP鄄1的阳性率为54.2%,明显高于正常大肠标本(P<0.05);MMP鄄9表达超过TIMP鄄1者,发生淋巴结转移和肝转移的比例明显高于TIMP鄄1表达超过MMP鄄9者。结论:MMP鄄9和TIMP鄄1表达失衡和大肠癌的浸润、淋巴结转移、肝转移密切相关,MMP鄄9有促进肿瘤侵袭转移的作用,TIMP鄄1和大肠癌的侵袭转移呈负相关;MMP鄄9表达超过TIMP鄄1者预后不良。

Study of Expression of Matrix Metalloproteinase-9 and Tissue Metalloproteinase Inhibitor-1 in Colorectal Carcinoma with Invasion Metastasis

Objective: To explore relationship of expression of matrix metalloproteinase-9 and tissue metalloproteinase inhibitor-1 in colorectal carcinoma to invasion and metastasis. Methods: By using SP immunohistochemical method, the expression of MMP-9 and TIMP-1 were detected in 48 cases of colorectal carcinoma specimen and 23 cases of normal colorectal specimen. Results: The positive rate of MMP-9 in colorectal carcinoma was 87.5% which was higher than that in normal colorectal specimen(P < 0.05); The positive rates of MMP-9 in patients with lymph node or liver metastasis were higher than that in patients with no lymph node or liver metastasis(P < 0.05); The positive rates of TIMP-1 in colorectal carcinoma were 54.2% which was different with that in normal colorectal specimen(P < 0.05); Lymph node and liver metastasis positive rates of the patients with the expression of MMP-9 over TIMP-1 were higher than patients with the expression of TIMP-1 over MMP-9. Conclusion: Expression unbalance of MMP-9 and TIMP-1 has a close relationship with lymph node or liver metastasis. MMP-9 promotes tumor metastasis, and TIMP-1 inhibits tumor metastasis. The patients with the expression of MMP-9 over TIMP-1 have poorer prognosis.