| 红藻氨酸致癫痫持续状态大鼠海马neuropilin-2 mRNA及其蛋白表达的研究 |
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| 引用本文： | 邓晓清,郑金瓯,余璐,陈子蓉.红藻氨酸致癫痫持续状态大鼠海马neuropilin-2 mRNA及其蛋白表达的研究[J].中风与神经疾病杂志,2010,27(10). |
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| 作者姓名： | 邓晓清 郑金瓯 余璐 陈子蓉 |
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| 作者单位： | 1. 广西南宁市第八人民医院神经内科,广西南宁,530001
2. 广西医科大学第一附属医院神经内科,广西南宁,530021 |
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| 基金项目： | 广西科学基金项目(桂科自0640118) |
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| 摘 要： | 目的研究轴索导向分子NPN-2mRNA及其蛋白对癫痫持续状态(SE)后大鼠海马内神经纤维外向性生长和突触重建中的调控作用。方法采用侧脑室内注射红藻氨酸(KA)制作TLE大鼠模型,用Nissl染色、原位杂交和免疫组织化学的方法,分别检测致SE后1d、1w、2w、3w、4w大鼠海马齿状回(DG)、CA1区、CA3区、门区神经元丢失程度以及NPN-2mRNA及其蛋白的表达。结果 KA致SE后1d开始出现神经元丢失,至4w神经元丢失明显增多。KA致SE后1d,NPN-2mRNA及其蛋白在DG和CA1区表达明显下降,持续至3w(P0.01),4w恢复至正常(P0.05);NPN-2mRNA及其蛋白在门区、CA3区表达实验组与对照组无明显差别(P0.05)。结论 KA致SE后,海马DG及CA1区神经元下调NPN-2mRNA及其蛋白的表达,促进DG及CA1区神经纤维外向性生长和突触的重建。
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| 关 键 词： | 红藻氨酸 癫痫持续状态 海马 Neuropilin-2 |
Altered expression of neuropilin-2 mRNA and protein in hippocampus after kainic-induced status epilepticus rats |
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| DENG Xiao-qing,ZHENG Jin-ou,YU Lu,et al..Altered expression of neuropilin-2 mRNA and protein in hippocampus after kainic-induced status epilepticus rats[J].Journal of Apoplexy and Nervous Diseases,2010,27(10). |
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| Authors: | DENG Xiao-qing ZHENG Jin-ou YU Lu |
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| Institution: | DENG Xiao-qing,ZHENG Jin-ou,YU Lu,et al.(Department of Neurology,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
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| Abstract: | Objective To study the guiding fuctions of axon guidance molecules,NPN-2 in axonal sprouting outgrowth and synaptic reorganization in hippocampus by detecting it's mRNA and protein expression in rats after kainic acid (KA)-induced status epilepticus (SE).Methods Adult rats were made to be a model of SE by intraventricular injection of KA.At 1d,1w,2w,3w and 4w after KA-induced SE,neurons loss were detected by Nissl staining,NPN-2 mRNA and protein expression in the dentate gyrus (DG),CA1,CA3 and hillus region... |
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| Keywords: | Kainic acid Status epilepticus Hippocampus Neuropilin-2 |
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