| 西洛他唑对糖氧剥离大鼠脑微血管内皮细胞的保护作用 |
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| 引用本文： | 吴云,王晓坤,梁庆成,张苏明,丛林. 西洛他唑对糖氧剥离大鼠脑微血管内皮细胞的保护作用[J]. 中风与神经疾病杂志, 2010, 27(10) |
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| 作者姓名： | 吴云 王晓坤 梁庆成 张苏明 丛林 |
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| 作者单位： | 1. 哈尔滨医科大学附属二院神经内科,黑龙江,哈尔滨,150086
2. 华中科技大学同济医学院,湖北,武汉,430030 |
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| 基金项目： | 2008年黑龙江省卫生厅科研课题资助(2007-333); 哈医大二院2007年院博士科研基金项目资助(BS2007-11) |
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| 摘 要： | 目的探讨磷酸二酯酶(PDE)3抑制剂西洛他唑对大鼠脑微血管内皮细胞糖氧剥离损伤的影响及作用机制。方法原代培养大鼠皮层微血管内皮细胞,建立糖氧剥离细胞模型模拟"缺血"过程,分5组:正常对照组、西洛他唑组、依达拉奉组、溶剂对照组、模型组。糖氧剥离6h后,测定细胞上清中内皮型一氧化氮合酶(eNOS)和诱导型一氧化氮合酶(iNOS)水平、细胞内环磷腺苷酸(cAMP)浓度,及采用四唑盐(MTT)比色实验测定细胞活力。结果西洛他唑及依达拉奉均可明显提高缺血细胞模型上清中eNOS水平,同时降低iNOS水平,提高细胞内cAMP水平及细胞存活率(均P0.05),且西洛他唑作用更为显著。结论西洛他唑对培养大鼠皮层微血管内皮细胞在缺血损伤中具有保护作用,其作用机制可能是通过选择性抑制PDE3从而增加cAMP水平,间接增加eNOS水平、降低iNOS水平来实现的。
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| 关 键 词： | 西洛他唑 脑微血管内皮细胞 糖氧剥离 保护 |
Protection of cilostazol on cultured brain microvascular endothelial cells deprived of oxygen and glucose in rats |
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| WU Yun,WANG Xiao-kun,LIANG Qing-cheng,et al.. Protection of cilostazol on cultured brain microvascular endothelial cells deprived of oxygen and glucose in rats[J]. Journal of Apoplexy and Nervous Diseases, 2010, 27(10) |
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| Authors: | WU Yun WANG Xiao-kun LIANG Qing-cheng et al. |
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| Affiliation: | WU Yun,WANG Xiao-kun,LIANG Qing-cheng,et al.(Department of Neurology,The Second Affiliated Hospital of Harbin Medical University,Harbin 150086,China). |
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| Abstract: | Objective To study the effects and possible mechanism of cilostazol on cultured brain microvascular endothelial cells deprived of oxygen and glucose in rats in vitro.Methods Brain microvascular endothelial cells of the Wistar rats were cultured in vitro,and then cell model of ischemia was established by oxygen-glucose deprivation (OGD) mimicking cerebral ischemia.All the cells were divided randomly into the normal group,cilostazol group,edaravone group,solvent group and OGD group.Concentrations of endotheli... |
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| Keywords: | Cilostazol Brain microvascular endothelial cells Oxygen-glucose deprivation Protection |
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