Characterization of a galactose-1-phosphate uridylyltransferase gene from the marine red alga Gracilaria gracilis |
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| Authors: | A. O. Lluisma M. A. Ragan |
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| Affiliation: | (1) Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1 and Department of Biology, Dalhousie University, Halifax, NS, Canada B3H 4J1, CA;(2) Institute for Marine Biosciences, National Research Council of Canada, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1 and Canadian Institute for Advanced Research in Evolutionary Biology e-mail: mark.ragan@nrc.ca Tel.: +1-902-426 1674 Fax: +1-902-426 9413, CA |
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| Abstract: | The metabolism of D-galactose is a major feature of red-algal physiology. We have cloned and sequenced a gene from the red alga Gracilaria gracilis that encodes a key enzyme of D-galactose metabolism, galactose-1-phosphate uridylyltransferase (GALT). This gene, designated GgGALT1, is apparently devoid of introns. A potential TATA box, four potential CAAT boxes, and a repeated sequence occur in the 5′-flanking region. The predicted 369-aa peptide shares significant sequence similarity with GALTs from other organisms (human, 47%; Saccharomyces cerevisiae, 49%; Solanum tuberosum, 49%). Southern-hybridization analysis reveals two related, but apparently not identical, GALT genes in the nuclear genome of G. gracilis. Sequence analysis indicates that the GgGALT1 enzyme lacks a rubredoxin “knuckle” motif, which in bacterial and fungal GALTs is involved in binding zinc. An open reading frame encoding a potential peptidyl tRNA hydrolase occurs 179 bp downstream from the GgGALT1 gene. Received: 6 April / 2 June 1998 |
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| Keywords: | Gracilaria gracilis Rhodophyceae Galactose metabolism Nuclear gene Uridylyltransferase |
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