| 人骨形成蛋白2的基因的克隆和序列分析 |
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| 引用本文： | 王德利,阮狄克,张惠中,石峥,范清宇. 人骨形成蛋白2的基因的克隆和序列分析[J]. 海军总医院学报, 2003, 16(1): 8-10 |
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| 作者姓名： | 王德利 阮狄克 张惠中 石峥 范清宇 |
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| 作者单位： | 1. 海军总医院骨科,北京,100037
2. 第四军医大学唐都医院骨研所,西安,710038 |
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| 摘 要： | 目的 克隆人骨形成蛋白2基因全长。方法 以成骨肉瘤细胞总RNA为模板，应用转录酶-多聚酶链反应法克隆人骨形成蛋白2的cDNA全长；将获得的基因插入pGEM—T—Easy载体质粒，并转化至大肠杆菌后挑选阳性克隆，利用限制性内切酶酶切分析鉴定重组质粒。结果 通过质粒酶切分析和序列测定，我们获得的基因为人骨形成蛋白2全长DNA序列。结论 克隆获得人骨形成蛋白2的基因，为其进一步开发利用提供了前提条件。
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| 关 键 词： | 骨形成蛋白 基因克隆 |
| 文章编号： | 1009-3427(2003)01-0008-03 |
Cloning of Human Bone Morphogenetic Protein 2 Gene |
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| WANG De-li,RUAN Di-ke,ZHANG Hui-zhong,et al. Cloning of Human Bone Morphogenetic Protein 2 Gene[J]. Journal of Naval General Hospital of PLA, 2003, 16(1): 8-10 |
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| Authors: | WANG De-li RUAN Di-ke ZHANG Hui-zhong et al |
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| Abstract: | Objective The purpose of this study is clone human BMP2 cDNA. Methods Using U2-OS cell to extract cell total RNA, human BMP2 cDNA was amplified by RT-PCR. The human BMP2 cDNA was then inserted into cloning vector pGEM-T-Easy. Screening by white and blue clone after transforming the recombinant plasmid into E. coli, we got the recombinant plasmid which was confirmed by restriction enzyme and sequence analysis. Results Restriction enzyme assay and sequenc e analysis showed that the construct we got was human BMP2 cDNA. Conclusion We obtained human BMP2 cDNA which provide us useful target for further study. |
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| Keywords: | Bone morphogenetic protein Gene cloning |
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