ERK信号转导通路在CdCl2诱导HEK293细胞低剂量兴奋效应中的作用

目的 探讨ERK信号转导通路在CdCl2诱导HEK293细胞低剂量兴奋效应过程中的作用.方法 以0、0.0005、0.005、0.05、0.5、5、50和500μmol/L浓度的氯化镉(CdCl2)分别染毒HEK293细胞12h和24h;在染毒前分别用ERK1/2抑制剂PD98059(100μmol/L)、U0126(...

Role of ERK in the hormesis induced by cadmium chloride in HEK293 cells

Objective To detect the role of ERK in the hormesis induced by cadmium chloride in HEK293 cells.Methods HEK293 cells were treated with CdCl2 of 0,0.0005,0.005,0.05,0.5,5,50,500μmol/L for 12h and 24h.The ERK inhibitors(PD98059,U0126) and c-raf shRNA were added before the cell was treated with CdCl2.The proliferation viability was measured by thethiazolyl blue(MTT) and WST-8 assay.The level of the phosphorylated ERK and c-raf was examined with the western blot method.The level of c-fos,c-myc and c-raf mRNA was examined with RT-PCR.Results CdCl2 stimulated cell proliferation at lower concentrations(0.05 and 0.5μmol/L) but inhibit it at higher concentrations(50 and 500μmol/L).The c-fos mRNA increased significantly at low concentrations(0.5μmol/L) and the c-myc mRNA increased with CdCl2 concentration.Treated with 100μmol/L ERK1/2 inhibitor and c-raf shRNA,the cell proliferation did not increase significantly at low concentrations(0.05 and 0.5μmol/L),but still decreased at high concentrations(50 and 500μmol/L).Conclusion CdCl2 can induce the hormesis of HEK293 cell proliferation.The c-fos of ERK pathway may be involved in the biphasic effect induced by CdCl2.