葛根芩连方药中小檗碱、药根碱和巴马汀的高效毛细管电泳分析

目的 以高效毛细管电泳法分离并测定葛根芩连方药中黄连生物碱。方法 通过毛细管区带电泳法，以60％的磷酸盐(60mmol／L，pH=8．0)与40％甲醇为缓冲液，苄基三乙基氯化铵为内标，紫外254nm检测，分离并测定葛根芩连汤水提物及微丸中小檗碱、巴马汀、药根碱的含量。结果 黄连中生物碱在6min内得到很好的分离，小檗碱、巴马汀、药根碱的加样回收率分别为99．05％、98．70％和97．76％；相对标准偏差(RSD)分别为2．12％、1．85％和1．95％。结论 该方法具有良好的精密席和回收率，可作为黄连制剂的研究提供含量测定方法。

High-performance capillary electrophoresis for determining the contents of berberine, jatrorrhizine and palmatine in Gegenqinlian decoction.

OBJECTIVE: To develop a method for alkaloid isolation from the extract of Gegenqinlian decoction (a traditional Chinese herbal preparation) and content determination. METHOD: After the preparation of the Gegenqinlian decoction extract and the micropellets, the quaternary alkaloids of Coptis chinensis were isolated by high-performance capillary electrophoresis (HPCE) in a buffer solution containing 60% sodium phosphate (60 mmol/L, pH 8.0) and 40% methanol. 2-(4-hydroxyphenyl) ethylammonium chloride was used as the internal standard and the ultraviolet detection was performed at 254 nm for determining the contents of berberine, jatrorrhizine and palmatine in the extract of Gegenqinlian decoction. RESULTS: Berberine, jatrorrhizine and palmatine were successfully isolated within 6 min with recovery rates of 99.05%, 98.70% and 97.76% respectively and relative standard deviation of 2.12%, 1.85% and 1.95% respectively. CONCLUSION: HPCE is applicable for the determination and isolation of quaternary alkaloids in Coptis chinensis with high accuracy and recovery rate.