刺五加胶囊对抑郁模型大鼠结肠组织iNOS、NO和COX-2的影响

①目的探讨刺五加胶囊对抑郁模型大鼠结肠组织的作用及其机制。②方法建立大鼠抑郁模型，予以刺五加胶囊（剂量300、600、900mg／kg）与5-氨基水杨酸（5-ASA，100mg／kg）灌肠给药2周后，采用生化方法检测大鼠结肠组织中诱导型一氧化氮合酶（iNOS）和一氧化氮（NO）的含量；用免疫组化方法检测结肠组织环氧合酶-2（COX-2）的表达。③结果抑郁模型大鼠结肠组织中iNOS和NO的含量均明显升高（F-21．266、11．741，q=5．543～9．841，P〈0．01），COX-2在抑郁大鼠结肠中表达增加（F-51．079，q=7．843，P〈0．01）；各剂量刺五加胶囊灌肠后iNOS和NO的含量降低（q=7．726～8．902，P〈0．01），600、900mg／kg刺五加胶囊灌肠后COX-2的表达明显降低（q=9．716～11．402，P〈0．05）。④结论刺五加胶囊可能通过下调抑郁模型大鼠结肠组织iNOS和NO含量及COX-2表达发挥对结肠的保护作用。

EFFECTS OF CIWUJIA CAPSULE ON INDUCIBLE NITRIC OXIDE SYNTHASE, NITRIC OXIDE AND CYCLOOXYGENASE-2 IN COLON OF DEPRESSION MODEL OF RATS

Objective To investigate the protective effects of Ciwujia capsule on the colon of depression model rats and its mechanisms. Methods The depression model of rat was produced. Ciwujia capsule and 5-ASA were administered by enema for two weeks. The inducible nitric oxide synthase (iNOS), nitric oxide (NO) and the expression of cyclooxygenase-2 (COX-2) in the colon were detected by corresponding kits and immunohistochemical technology . Results Depression increased significantly the levels of iNOS, NO and the expression of COX-2; Ciwujia capsule (300,600,900 mg/kg)could down-regulate the levels of iNOS and NO. At dose of 600,900 mg/kg ,the capsules could decrease the expression of COX-2. Conclusion Ciwujia capsule can protect the colon of depressed rats by decreasing the levels of iNOS, NO and the expression of COX-2 in colon.